ANSWERS|LATEST !!!!2026|GRADED A+| (EXAM
If you are working in a lab in which an autoclave is not available, and you are pressed for
time, which would you choose to best sterilize your equipment? Hot steam or hot air?
Explain why you chose your answer. - ANSWER Hot steam is the best choice as you
can achieve a sterile environment in a matter of minutes whereas hot air will take several
hours to achieve the same effect.
What three elements are used in an autoclave to sterilize equipment? -
ANSWER Heat, pressure, and steam.
What type of incubator is pictured below? - ANSWER Fixed incubator
What is the minimum temperature an autoclave must be set at to achieve sterile condition?
- ANSWER 125°C
At what temperature is the fixed incubator set to, as presented in the lab video? -
ANSWER 37°C
What are the FOUR types of gloves presented in the lab video? - ANSWER Latex,
Nitrile, Thermal cold, Thermal heat
What are the main sections that should be found in a lab notebook? Name at least 4. -
ANSWER Objective, Procedure, Notes, Results and Deviations
1
, At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.
- ANSWER 4°C. This temperature slows bacterial growth and prolongs the life of the
sample.
What THREE rules were discussed in regards to lab safety that would protect you and others
from contamination? - ANSWER 1. Never eat or drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
You are a lab instructor, and Sally Miller has turned in her lab notebook for you to grade the
lab experiment #1 on lab safety. Based upon what was covered in the lab video, how should
Sally have titled her experiment? - ANSWER SM01 Lab Safety
You arrive to your first day of work at a new lab. You are taking over for someone who took a
new job at another lab. Your boss informs you that because of time restraints, this person
did not exactly follow the experimental protocol. In order to proceed, you must know what
he did differently. (1) According to the lab lecture, under what section of his notebook would
you look to find the experimental steps? (2) As changes to the experimental steps were
made, what are these differences called, and how should it appear in the lab notebook? -
ANSWER (1) Procedure—this is where the steps for the experimental protocol are
recorded.
(2) Deviations. All deviations should be written in red to immediately bring attention to the
changes in the protocol.
1. Identify the part of the microscope indicated by the arrow. - ANSWER Neck/arm
2. Identify the part of the microscope indicated by the arrow. - ANSWER stage
3. Identify the part of the microscope indicated by the arrow. - ANSWER fine focus
2
If you are working in a lab in which an autoclave is not available, and you are pressed for
time, which would you choose to best sterilize your equipment? Hot steam or hot air?
Explain why you chose your answer. - ANSWER Hot steam is the best choice as you
can achieve a sterile environment in a matter of minutes whereas hot air will take several
hours to achieve the same effect.
What three elements are used in an autoclave to sterilize equipment? -
ANSWER Heat, pressure, and steam.
What type of incubator is pictured below? - ANSWER Fixed incubator
What is the minimum temperature an autoclave must be set at to achieve sterile condition?
- ANSWER 125°C
At what temperature is the fixed incubator set to, as presented in the lab video? -
ANSWER 37°C
What are the FOUR types of gloves presented in the lab video? - ANSWER Latex,
Nitrile, Thermal cold, Thermal heat
What are the main sections that should be found in a lab notebook? Name at least 4. -
ANSWER Objective, Procedure, Notes, Results and Deviations
1
, At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.
- ANSWER 4°C. This temperature slows bacterial growth and prolongs the life of the
sample.
What THREE rules were discussed in regards to lab safety that would protect you and others
from contamination? - ANSWER 1. Never eat or drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
You are a lab instructor, and Sally Miller has turned in her lab notebook for you to grade the
lab experiment #1 on lab safety. Based upon what was covered in the lab video, how should
Sally have titled her experiment? - ANSWER SM01 Lab Safety
You arrive to your first day of work at a new lab. You are taking over for someone who took a
new job at another lab. Your boss informs you that because of time restraints, this person
did not exactly follow the experimental protocol. In order to proceed, you must know what
he did differently. (1) According to the lab lecture, under what section of his notebook would
you look to find the experimental steps? (2) As changes to the experimental steps were
made, what are these differences called, and how should it appear in the lab notebook? -
ANSWER (1) Procedure—this is where the steps for the experimental protocol are
recorded.
(2) Deviations. All deviations should be written in red to immediately bring attention to the
changes in the protocol.
1. Identify the part of the microscope indicated by the arrow. - ANSWER Neck/arm
2. Identify the part of the microscope indicated by the arrow. - ANSWER stage
3. Identify the part of the microscope indicated by the arrow. - ANSWER fine focus
2
