Biochemistry ACS Exam
Questions and Answers 100%
PASS
what are nucleotides—ANSWER-monomer of nucleic acids, consists of N
base, 5-carbon sugar, and phosphate group
DNA—ANSWER-carry genetic information, double-stranded, more stable
RNA—ANSWER-intermediate of gene expression, single-stranded, less stable
tRNA—ANSWER-transfer RNA, holds amino acid for protein synthesis
wobble position—ANSWER-looser binding, pairing of 3rd codon less critical
inosine—ANSWER-closely resembles G, can pair with A, U, or C, allows
tRNA flexibility
rRNA—ANSWER-ribosome RNA, makes up ribosomes
siRNA—ANSWER-small interfering RNA, double-stranded, mRNA
degradation
miRNA—ANSWER-micro RNA, silencing and post-transcriptional regulation
ribozyme—ANSWER-ribosomes catalyze peptide bond formation
purines—ANSWER-2 rings, adenine(NH), guanine (O)
, pyramidines—ANSWER-1 ring, cytosine (NH), uracil (O), thymine (CH3)
A-T/U—ANSWER-2 H bonds
G-C—ANSWER-3 H bonds
Tm—ANSWER-temp at which 50% of DNA is annealed, GC higher than AT
A DNA—ANSWER-short and wide, 11bp, cylindrical, dehydrated, right
handed, angled base pairs, weaker H bonds
B DNA—ANSWER-10bp, right handed, parallel base pairs, strong H bonds
Z DNA—ANSWER-long and narrow, 12bp, left handed, GC rich
REN—ANSWER-restriction endonuclease recombination
PCR—ANSWER-polymerase chain reaction, denaturing (95C), anneal (65C),
extend (72C)
Sanger sequencing—ANSWER-dideoxyNTP (ddNTP), causes DNA polymerase
to stop, color labeled
Helicase—ANSWER-unwind, ATP dependent
single stranded binding protein—ANSWER-prevents reannealing
DNA poly I—ANSWER-removes primers
DNA poly II—ANSWER-repairs
DNA poly III—ANSWER-polymerization
DNA poly α—ANSWER-initiation
DNA poly δ—ANSWER-lagging
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law
Questions and Answers 100%
PASS
what are nucleotides—ANSWER-monomer of nucleic acids, consists of N
base, 5-carbon sugar, and phosphate group
DNA—ANSWER-carry genetic information, double-stranded, more stable
RNA—ANSWER-intermediate of gene expression, single-stranded, less stable
tRNA—ANSWER-transfer RNA, holds amino acid for protein synthesis
wobble position—ANSWER-looser binding, pairing of 3rd codon less critical
inosine—ANSWER-closely resembles G, can pair with A, U, or C, allows
tRNA flexibility
rRNA—ANSWER-ribosome RNA, makes up ribosomes
siRNA—ANSWER-small interfering RNA, double-stranded, mRNA
degradation
miRNA—ANSWER-micro RNA, silencing and post-transcriptional regulation
ribozyme—ANSWER-ribosomes catalyze peptide bond formation
purines—ANSWER-2 rings, adenine(NH), guanine (O)
, pyramidines—ANSWER-1 ring, cytosine (NH), uracil (O), thymine (CH3)
A-T/U—ANSWER-2 H bonds
G-C—ANSWER-3 H bonds
Tm—ANSWER-temp at which 50% of DNA is annealed, GC higher than AT
A DNA—ANSWER-short and wide, 11bp, cylindrical, dehydrated, right
handed, angled base pairs, weaker H bonds
B DNA—ANSWER-10bp, right handed, parallel base pairs, strong H bonds
Z DNA—ANSWER-long and narrow, 12bp, left handed, GC rich
REN—ANSWER-restriction endonuclease recombination
PCR—ANSWER-polymerase chain reaction, denaturing (95C), anneal (65C),
extend (72C)
Sanger sequencing—ANSWER-dideoxyNTP (ddNTP), causes DNA polymerase
to stop, color labeled
Helicase—ANSWER-unwind, ATP dependent
single stranded binding protein—ANSWER-prevents reannealing
DNA poly I—ANSWER-removes primers
DNA poly II—ANSWER-repairs
DNA poly III—ANSWER-polymerization
DNA poly α—ANSWER-initiation
DNA poly δ—ANSWER-lagging
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law
