ASCP MLT EXAM FINAL PAPER 2026 FULL
STUDY GUIDE
◉ A;
In DNA complementary base pairing, guanine and cytosine pair and
adenine and thymine base pair. Remembering the phrase "G-CAT"
helps one recollect correct pairing. Answer: Which nitrogen base
would bind with a guanine nucleotide in forming double-stranded
DNA?
A. Cytosine
B. Uracil
C. Thymine
D. Adenine
◉ B;
To make a 1 : 10 dilution, 270 µL of diluent should be added to 30 µL
of sample, . Answer: A spinal fluid that is slightly hazy is briefly
examined microscopically. The technologist performing the count
decides to make a 1:10 dilution using 30 µL of sample. What volume
of diluent should be used?
A. 70 µL
,B. 270 µL
C. 300 µL
◉ D;
Intravascular hemolysis is typically associated with increased levels
of serum (plasma) LDH and bilirubin, and an increased number of
reticulocytes. Serum LDH is found in higher levels during
intravascular hemolysis due to fact that high levels of LDH are
normally found within the red cells, but is now being spilled into the
bloodstream via red cell lysis. Bilirubin is a breakdown product of
hemoglobin, which has also been spilled into the bloodstream from
broken red blood cells via hemolysis. Reticulocytosis is a reflection
of the release of an increased number of immature red blood cells
from the marrow to account for the red blood cells that are lost
through hemolysis. This is a normal response. Failure to show an
increased reticulocyte count with hemolytic episodes or hemorrhage
would indicate an ineffective erythropoiesis (possible bone marrow
function problem). Answer: Which of these blood levels will increase
during intravascular hemolysis?
A. Serum (plasma) LDH
B. Serum (plasma) bilirubin
C. Reticulocytes
D. All of the above
E. None of the above
,◉ True
According to the paper by Jedrzejas, S. pneumoniae indeed is the
cause of most fatal cases of community-acquired pneumonia.
Answer: True/False
Streptococcus pneumoniae is the most common cause of fatal
community-acquired pneumonia.
◉ C;
A slow, progressive drift of values away from the mean is called a
trend. Trends occur when a particular analyte value slowly and
progressively changes in one direction from one run to the next.
Monitoring data for trends is part of good laboratory practice and is
one indicator of many that helps laboratorians determine whether
the measurement system is fucntioning appropriately. Answer: A
slow, progressive drift of values away from the mean is called a:
A. Coefficient of Deviation
B. Dispersion
C. Trend
D. Variance
E. Shift
◉ C;
, Protein in urine can be confirmed using sulfosalicylic acid (SSA)
precipitation. The SSA reagent is added to a small volume of urine.
Acidification causes precipitation of protein in the sample, which is
subjectively graded as trace, 1+, 2+, 3+ or 4+. SSA reaction will
detect albumin, globulins, and Bence-Jones proteins. Answer: Which
of the following would be the most appropriate method to confirm a
positive protein from a urine dipstick:
A. Immunoelectropheresis
B. Heat precipitation
C. Sulfosalicylic acid precipitation
D. Protein electrophoresis
◉ C;
Patients who develop severe sepsis or septic shock commonly have
increased plasma lactic acid values. Answer: Patients who develop
severe sepsis or septic shock commonly have __________ plasma lactic
acid values.
A. Decreased
B. Normal
C. Increased
◉ B;
STUDY GUIDE
◉ A;
In DNA complementary base pairing, guanine and cytosine pair and
adenine and thymine base pair. Remembering the phrase "G-CAT"
helps one recollect correct pairing. Answer: Which nitrogen base
would bind with a guanine nucleotide in forming double-stranded
DNA?
A. Cytosine
B. Uracil
C. Thymine
D. Adenine
◉ B;
To make a 1 : 10 dilution, 270 µL of diluent should be added to 30 µL
of sample, . Answer: A spinal fluid that is slightly hazy is briefly
examined microscopically. The technologist performing the count
decides to make a 1:10 dilution using 30 µL of sample. What volume
of diluent should be used?
A. 70 µL
,B. 270 µL
C. 300 µL
◉ D;
Intravascular hemolysis is typically associated with increased levels
of serum (plasma) LDH and bilirubin, and an increased number of
reticulocytes. Serum LDH is found in higher levels during
intravascular hemolysis due to fact that high levels of LDH are
normally found within the red cells, but is now being spilled into the
bloodstream via red cell lysis. Bilirubin is a breakdown product of
hemoglobin, which has also been spilled into the bloodstream from
broken red blood cells via hemolysis. Reticulocytosis is a reflection
of the release of an increased number of immature red blood cells
from the marrow to account for the red blood cells that are lost
through hemolysis. This is a normal response. Failure to show an
increased reticulocyte count with hemolytic episodes or hemorrhage
would indicate an ineffective erythropoiesis (possible bone marrow
function problem). Answer: Which of these blood levels will increase
during intravascular hemolysis?
A. Serum (plasma) LDH
B. Serum (plasma) bilirubin
C. Reticulocytes
D. All of the above
E. None of the above
,◉ True
According to the paper by Jedrzejas, S. pneumoniae indeed is the
cause of most fatal cases of community-acquired pneumonia.
Answer: True/False
Streptococcus pneumoniae is the most common cause of fatal
community-acquired pneumonia.
◉ C;
A slow, progressive drift of values away from the mean is called a
trend. Trends occur when a particular analyte value slowly and
progressively changes in one direction from one run to the next.
Monitoring data for trends is part of good laboratory practice and is
one indicator of many that helps laboratorians determine whether
the measurement system is fucntioning appropriately. Answer: A
slow, progressive drift of values away from the mean is called a:
A. Coefficient of Deviation
B. Dispersion
C. Trend
D. Variance
E. Shift
◉ C;
, Protein in urine can be confirmed using sulfosalicylic acid (SSA)
precipitation. The SSA reagent is added to a small volume of urine.
Acidification causes precipitation of protein in the sample, which is
subjectively graded as trace, 1+, 2+, 3+ or 4+. SSA reaction will
detect albumin, globulins, and Bence-Jones proteins. Answer: Which
of the following would be the most appropriate method to confirm a
positive protein from a urine dipstick:
A. Immunoelectropheresis
B. Heat precipitation
C. Sulfosalicylic acid precipitation
D. Protein electrophoresis
◉ C;
Patients who develop severe sepsis or septic shock commonly have
increased plasma lactic acid values. Answer: Patients who develop
severe sepsis or septic shock commonly have __________ plasma lactic
acid values.
A. Decreased
B. Normal
C. Increased
◉ B;
