ASCP Molecular Biology Certification Exam with Questions and Answers

ASCP Molecular Biology Certification Exam with Questions and Answers What is direct analysis? ANSWER identifying a specific gene or mutation that caues disease. gene must be known, might need to know mut What are some direct analysis methods? ANSWER southern PCR - ASO blot, restriction digest SSCP HA What some indirect analysis methods? ANSWER RFLP linkage analysis What is indirect analysis? ANSWER inherited marker near gene associated with disease unknown gene and /or mut Describe the growth of the nucleic acid chain ANSWER The chain grows by the attachment of the 5' phosphate group of an incoming nucleotide to the 3' hydroxyl group of the last nucleotide on the growing chain Denaturing agents ANSWER formamide, urea, mercaptoethanol Histones ANSWER Proteins that DNA tightly coils around to form chromosomes Octamer, 2 ea of: H2A, H2B, H3, and H4 Histones are hydrophobic (basic K & R,+) DNA is hydrophilic (P backbone, -) +/- interaction keeps them bound Solenoid ANSWER a coil of six nucleosomes wound into a tightly packed helix Mutation ANSWER DNA sequence change that is present in a relatively small proportion of the population <1%, somatic changes