1
Portage Microbiology Exam #5 Questions with
Correct Answers | Updated (100% Correct
Answers)
Define the concept of universal precautions (short answer)—
Answer: Universal precautions means any and all samples, whether
known or unknown, are to be treated as potentially hazardous (or
pathogenic) materials
List at lease 3 observations a researcher would be sure to note while
assessing and unknown microbial sample (short answer)— Answer:
Need to note:
1. Size and shape
2. Any observable motility
3. Gram status (positive or negative)
While observing an unknown sample of limited amounts, a
researcher must determine the following observations: 1) the
presence of any motility and 2) its Gram status using the same
sample- the liquid sample cannot be divided. Which would you
determine first and why? (short answer)— Answer: You must
determine motility before determining Gram status. Motility requires
a wet mount, while gram staining requires heat fixing the sample. If
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, 2
one were to begin with the gram stain, the heat fixation process
would kill the organism, making any observations regarding motility
impossible. The correct approach would be to place the liquid
culture on a glass slide and determine its motility status. Next, the
same liquid culture can be heat fixed and gram stained.
A facultative anaerobe is a microorganism capable of growth under
what conditions? (short answer)— Answer: A facultative anaerobe is
capable of growth under aerobic (with oxygen) and anaerobic
(without oxygen) conditions
True or False: The Lancefield groups are used to subdivide antigenic
groups of alpha-hemolytic Streptococcus— Answer: False. The
Lancefield groups are used to subdivide beta-hemolytic strep
The distinctions for Lancefield subgroups lie in its: (select all that
apply)
A. Catalase activity
B. Carbohydrate composition of antigens
C. Hemolytic activity
D. All of the above— Answer: B. Carbohydrate composition of
antigens
© 2025 All rights reserved
Portage Microbiology Exam #5 Questions with
Correct Answers | Updated (100% Correct
Answers)
Define the concept of universal precautions (short answer)—
Answer: Universal precautions means any and all samples, whether
known or unknown, are to be treated as potentially hazardous (or
pathogenic) materials
List at lease 3 observations a researcher would be sure to note while
assessing and unknown microbial sample (short answer)— Answer:
Need to note:
1. Size and shape
2. Any observable motility
3. Gram status (positive or negative)
While observing an unknown sample of limited amounts, a
researcher must determine the following observations: 1) the
presence of any motility and 2) its Gram status using the same
sample- the liquid sample cannot be divided. Which would you
determine first and why? (short answer)— Answer: You must
determine motility before determining Gram status. Motility requires
a wet mount, while gram staining requires heat fixing the sample. If
© 2025 All rights reserved
, 2
one were to begin with the gram stain, the heat fixation process
would kill the organism, making any observations regarding motility
impossible. The correct approach would be to place the liquid
culture on a glass slide and determine its motility status. Next, the
same liquid culture can be heat fixed and gram stained.
A facultative anaerobe is a microorganism capable of growth under
what conditions? (short answer)— Answer: A facultative anaerobe is
capable of growth under aerobic (with oxygen) and anaerobic
(without oxygen) conditions
True or False: The Lancefield groups are used to subdivide antigenic
groups of alpha-hemolytic Streptococcus— Answer: False. The
Lancefield groups are used to subdivide beta-hemolytic strep
The distinctions for Lancefield subgroups lie in its: (select all that
apply)
A. Catalase activity
B. Carbohydrate composition of antigens
C. Hemolytic activity
D. All of the above— Answer: B. Carbohydrate composition of
antigens
© 2025 All rights reserved
