1/
BIOLOGY 306 -FALL 2024 1V' ~
Quiz2
«~ :1 l"VJ.I
GEO RG E MA SO N
UNI VER SIT Y~
Name:
Date: \ 0 I ,o / 1.L-i
. . . ( m) Show your cal lation. (2
1n micrometer u •
s "=> =- "W
I. Using the image below, calibration the ocular (eyepiece)
OD= Y'S
pt)
40
~
/iiII/111I/ill! IIll~Ill/1111/1111/1111/1111II111/1111/11111111/1111ii1111111~IllIII111i1IllIIIii
/1111/IIII 1111/1111 11111111 11111111 1111/llll/ltll/llll/ttlf/llll
2. Identify the shape of each bacterium below: (1.5 points)
'
..'
,.-
~.
',1~ -'•;~ ·-.
,:.'-...,·-,
.--;~r;;.--- ...
• '
·~
y~
~~f
'
- -~
•
•
~
:-....___,""
•
..f..l. , , •• '
I
/
• • '-.
.,.
·~-
---✓-..____,,
--- ,..
l ,a
.
~ -.•.
,c.. 1· T,
••.
...
- • -
., .,·
' .••••
,
◄
1
Cocc.u~ ~c, \\05 Sp, vi\\~
VO lAlf\d • V'O.-~J'6
dye to use to visualize bacteria? (0.5 point)
3. Methlyene blue is a positively charged dye. Why is this a good
"V-~ ..~V't 11 £. VI c:. ~~ so .\vu. d.-j -e.. / d 1,-(y t ~
'Oeu.1.>-'.JI- 'oo. t.-\-t. ;o. ·, $
~ ~f
~J
\
4. Fill in the blanks: (2.5 points) f 7,,,,
EMB media is selecti_ve b~~
it inhi?its the gr~wth of gram- f'O ~i~ bacteria. The prese
n~e of
nt lactose Will appear w•U v,-~ n color on
lactose also makes this mecba dtfferenllal. Orgarnsms t!)af ferme
organisms that cannot erment lactose appear
the media; we r fermenter., will appear ~~ / , while
co\o✓ \t~ . ,.of/ ~~ /
..,,e ffr;~ organisms (the ones that can
MacConk~y agar_al~o con13!ns lact?se and is selJctive for gram-
1n the media that chc19ges color
grow). This mecha 1s also differential because of the ~~ •
m~ wi ll appear Vee),✓ .
depending on pH. If the pH is below 6.8, the colonies~that fer If t
_ __ , which is a pH indicator.
Mannitol salt agar is differential because of the presence of \~'1~ to 'I e~w .
from V:f:«jc
colonies growing on the plate produce acid, the media changes color
./
BIOLOGY 306 -FALL 2024 1V' ~
Quiz2
«~ :1 l"VJ.I
GEO RG E MA SO N
UNI VER SIT Y~
Name:
Date: \ 0 I ,o / 1.L-i
. . . ( m) Show your cal lation. (2
1n micrometer u •
s "=> =- "W
I. Using the image below, calibration the ocular (eyepiece)
OD= Y'S
pt)
40
~
/iiII/111I/ill! IIll~Ill/1111/1111/1111/1111II111/1111/11111111/1111ii1111111~IllIII111i1IllIIIii
/1111/IIII 1111/1111 11111111 11111111 1111/llll/ltll/llll/ttlf/llll
2. Identify the shape of each bacterium below: (1.5 points)
'
..'
,.-
~.
',1~ -'•;~ ·-.
,:.'-...,·-,
.--;~r;;.--- ...
• '
·~
y~
~~f
'
- -~
•
•
~
:-....___,""
•
..f..l. , , •• '
I
/
• • '-.
.,.
·~-
---✓-..____,,
--- ,..
l ,a
.
~ -.•.
,c.. 1· T,
••.
...
- • -
., .,·
' .••••
,
◄
1
Cocc.u~ ~c, \\05 Sp, vi\\~
VO lAlf\d • V'O.-~J'6
dye to use to visualize bacteria? (0.5 point)
3. Methlyene blue is a positively charged dye. Why is this a good
"V-~ ..~V't 11 £. VI c:. ~~ so .\vu. d.-j -e.. / d 1,-(y t ~
'Oeu.1.>-'.JI- 'oo. t.-\-t. ;o. ·, $
~ ~f
~J
\
4. Fill in the blanks: (2.5 points) f 7,,,,
EMB media is selecti_ve b~~
it inhi?its the gr~wth of gram- f'O ~i~ bacteria. The prese
n~e of
nt lactose Will appear w•U v,-~ n color on
lactose also makes this mecba dtfferenllal. Orgarnsms t!)af ferme
organisms that cannot erment lactose appear
the media; we r fermenter., will appear ~~ / , while
co\o✓ \t~ . ,.of/ ~~ /
..,,e ffr;~ organisms (the ones that can
MacConk~y agar_al~o con13!ns lact?se and is selJctive for gram-
1n the media that chc19ges color
grow). This mecha 1s also differential because of the ~~ •
m~ wi ll appear Vee),✓ .
depending on pH. If the pH is below 6.8, the colonies~that fer If t
_ __ , which is a pH indicator.
Mannitol salt agar is differential because of the presence of \~'1~ to 'I e~w .
from V:f:«jc
colonies growing on the plate produce acid, the media changes color
./
