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GMU BIO 306 Lab Quiz -1 2025

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GMU BIO 306 Lab Quiz -1 2025 Techniques of Microorganisms Lab Book

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Name:
Date: 02/19/2025
Sectlo
Total Point-15
Qulz-1

lQ.? Yo~ ~~e obs_erving a specimen under a microscope, and it spans ~ivisions. If 10
ocular d1v1s1on aligns with 25 t d" · · d - -=-----=---.....___ - -
. s age 1v1s10.n an_ _1 stage
__ division=
__ _ 10 µm ' then how long is the
_.._
specimen? (2 Point)
,o co :: i.s so 10 1s so x ,ov~ ~ '2..SO _
00
I
-
SO ::: 10 U W'1

so
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lO =-
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~Q. Yo~ rubbed ~our fingers on two agar plates before and after hand washing. After
so A "'" @1
incubation at 37 C for 24 hours, you found similar types of bacterial colonies. Which kind of
flora it can be?Pfeaseexplain. (1 Points). ~ ½ \,)0tnJ ·
1 ( ~s; (A'\'t f \~'(0, . I ':i i ~ ~1 pe Of b~ Cttv; ~ 1"'~\ (()'(' Qt ,{"" Q\/ ~ v-1~ (\,;,,,


.f,lov '\ 1) so evtn


\....~"' ~ v-1 ~\ n·•"'~ >"t.S·1 ~f b~ t .\-v-.-ia, I¼. c.olo"; r~ ~~\ o-"'/l -.\~
~\VI~ -t"V'I .\- w L\:i f1/ \J \. ~ ~ \,~ 'ttrt, ~ OI"- i\ V'' c. l h, l"1
- , I\JI , l,.t.. ~-. {µ,..,.J--
1--t s, ~+ ev -th•v.•
~ - ---:-~,----
- a=rr=,e-r. (1 Point)
na=a-ry...,..,b
3 Q. Give an example of Primary barrier and an example of seco---
f r ·• VN""'/ 8ti r1 ) e,,.r - ~ , 0v t,-S

~-t (P V\ d.-t'i if\ 'Yi ~tr - ( t(\ ,J-,-r Iled ~ ( u Vi '?...H'-l s
4Q. You are working ~ obaderium tuberculosis ~teria which may cause serious le.!!!,ru
disease because Qfjnbalatioo. What Biosatety level iab do you think will be good for handling
thisorganism?(lPoint) d ·+- C h .tti '-•Al.\.~,i i"J ;v ',vql!
N01 17>~ 1. bt(Cl'JS~ ors ~ '1V5-t Dtlm crv r\Q\ 1


U4\~ C\ "~ 15 i ()SC,~~ lel\Je l ~ bov(, -1._, (!sL 3)
SQ. We know that agar has no nutritive value for most bacteria. Then, what is the reasoning>
using agar in media? (1 points)

Be cav~ f>ija-.. ;) l\ j ood ;nevi:<~~t ""'bv";"' Cl Is o
~y



~ -\\ f ',Vt • ""J.. 't ~ t, ~ h.tJ Y\'\I +,, / -\,'-fY\ ~ \


he\iN" Blc ~ ~V\ r ~'., q Jol;cl~fe~ P\9e"➔• 54-lb't ,
6Q. J'Media are sterilized in the autoclave a ~ - -- psi, 121°C for 15-20 minutes. (1 point)


f~ y
~ 12-1 'c,

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