SBB UPDATED EXAM SCRIPT QUESTIONS AND ANSWERS
GRADED A+
✔✔What is the storage time if additive used is CPDA-1? - ✔✔35 days
✔✔What is the storage time if additive used is AS-1 (Adsol)? - ✔✔42 days
✔✔What is the storage time if additive used is AS-3 (nutricel)? - ✔✔42 days
✔✔What is the storage time if additive used is AS-5 (Optisol)? - ✔✔42 days
✔✔Whole Blood storage temp and description - ✔✔1-6C
contains all blood elements plus the anticoagulant-preservative commonly used as a
source for component production
✔✔RBC storage and description - ✔✔1-6C
Whole blood with plasma removed
RBCs without additives must have final hct <80%
✔✔Frozen RBC storage and description - ✔✔-65C or colder
frozen within 6 days if no additive solution
RBC w/additive must be frozen before expiration
must be put in freezer within 4 hours of removed from 4C
✔✔Rejuvenated RBC description - ✔✔rejuvenation should restore 2,3-DPG to 75% of
normal level and ATP above normal levels.
If done before 14 days may cause high 2,3-DPG which impairs O2 up-take.
AS-3 and AS-5 cannot be rejuvenated
✔✔Deglyced RBCs storage and description - ✔✔37C warmer or waterbath (20-25
minutes)
washed with solutions of decreasing osmolarity to avoid hemolysis.
must remove cryoprotectant agents, minimial free hgb, and result in recovery of at least
80% RBC volume.
open system:1-6C with 24 hr expiration.
closed system: 14 days or as FDA approved
✔✔What should the osmolality of a deglyced unit be? - ✔✔<500.
Osmolality is a fxn of free glycerol therefore an increase is a result of improper
deglycing
✔✔What should free hgb in last wash be from decglyed unit? - ✔✔<200
✔✔Washed RBCs storage - ✔✔1-6C
,washed with sterile normal saline to remove most of plasma
24 hour expiration
✔✔FFP storage and description - ✔✔prepared from WB or collected using apheresis.
frozen at -18C or below
contains max levels of labile or nonlabile coag factors
Thaw between 30-37C
✔✔Thawed plasma - ✔✔FFP thawed but not transfused within 24 hrs
can be stored 1-6C for 5 days
reduction in Factors V and VII
✔✔Liquid plasma - ✔✔plasma separated from WB at any time during storage, up to 5
days after the expiration date of WB. supernatant levels of potassium and ammonia are
increased
✔✔Cryo - ✔✔prepared from WB collected into CPDA-1 or CPD
precipitates when FFP thawed between 1-6C
stored at -18C or colder
suspended in less than 15mL plasma
contains factor VII and fibrinogen and most of XIII
single unit store at 20-24 for 6 hrs
pooled store at 20-24 for 4 hours
✔✔platelets - ✔✔prepared from WB that hasn't cooled below 20C
separated within 4 hours
soft spin (plt rich plasma) followed by a hard spin (platelet concentrate)
resuspended in about 45-65mL plasma
20-24C with gentle agitation
✔✔Granulocytes - ✔✔usually harvested in buffy coats
collection must yield 1.0x10 to the 10th granulocytes
Transfuse ASAP
✔✔Irradiation - ✔✔prevents proliferation of transfused T-Lymphs, the primary cause of
graft vs host (GVHD)
✔✔effects of storage on blood components - ✔✔decreases 2,3-DPG levels: this
controls release of O2 from RBCs, levels recover after 24 hrs post txn
decreases ATP
increases potassium
✔✔Leukocyte reduction must reduce WBC count to what? - ✔✔<5x10 to the sixth in at
least 95% of units samples and yield >85% RBC recovery
, ✔✔What must the minimum mean volume of Apheresis RBCs be? - ✔✔180mL packed
RBC volume per unit (>60g hgb)
95% of samples units must have at least 150mL (>50g hgb)
✔✔What must the minimum mean volume of Apheresis RBCs leukoreduced be? -
✔✔<5x10 to the sixth WBC and mean volume of 153mL packed RBC volume per unit
(>51g Hgb)
95% of sample units have at least 128mL (>42.5g hgb)
✔✔How much coag factor VIII and fibrinogen must cryo have? - ✔✔80IU Factor VIII and
150mg fibrinogen
pooled units must have same amt multipled by number of units in pool
✔✔What are Mendel's principles? - ✔✔random segregation: distinct genes are
randomly inherited one from each parent
✔✔what is independent assortmant (Mendel's principle)? - ✔✔genes inherited
independently if carried on different chromosomes.
combos of genes are not dependent on other genes (exception: linkage)
✔✔what is an autosome? - ✔✔any chromosome other than the sex chromosomes
✔✔What is meiosis? - ✔✔process by which sex cells are produced which have only one
copy of each chromosome (haploid).
✔✔What is mitosis? - ✔✔process where sex cells are duplicated and equally divided
between two daughter cells
✔✔zygote - ✔✔fertilized egg
✔✔gamete - ✔✔sex cells....egg and sperm
✔✔diploid - ✔✔normal number of chromosomes in zygote and all body cells (46)
✔✔haploid - ✔✔normal number of chromosomes in all gametes (23)
✔✔structural gene - ✔✔gene that controls the protein production of another gene or
genes
✔✔regulator gene - ✔✔gene that controls the rated of production of another gene or
genes
✔✔allele - ✔✔alternate forms of a gene at a given locus
GRADED A+
✔✔What is the storage time if additive used is CPDA-1? - ✔✔35 days
✔✔What is the storage time if additive used is AS-1 (Adsol)? - ✔✔42 days
✔✔What is the storage time if additive used is AS-3 (nutricel)? - ✔✔42 days
✔✔What is the storage time if additive used is AS-5 (Optisol)? - ✔✔42 days
✔✔Whole Blood storage temp and description - ✔✔1-6C
contains all blood elements plus the anticoagulant-preservative commonly used as a
source for component production
✔✔RBC storage and description - ✔✔1-6C
Whole blood with plasma removed
RBCs without additives must have final hct <80%
✔✔Frozen RBC storage and description - ✔✔-65C or colder
frozen within 6 days if no additive solution
RBC w/additive must be frozen before expiration
must be put in freezer within 4 hours of removed from 4C
✔✔Rejuvenated RBC description - ✔✔rejuvenation should restore 2,3-DPG to 75% of
normal level and ATP above normal levels.
If done before 14 days may cause high 2,3-DPG which impairs O2 up-take.
AS-3 and AS-5 cannot be rejuvenated
✔✔Deglyced RBCs storage and description - ✔✔37C warmer or waterbath (20-25
minutes)
washed with solutions of decreasing osmolarity to avoid hemolysis.
must remove cryoprotectant agents, minimial free hgb, and result in recovery of at least
80% RBC volume.
open system:1-6C with 24 hr expiration.
closed system: 14 days or as FDA approved
✔✔What should the osmolality of a deglyced unit be? - ✔✔<500.
Osmolality is a fxn of free glycerol therefore an increase is a result of improper
deglycing
✔✔What should free hgb in last wash be from decglyed unit? - ✔✔<200
✔✔Washed RBCs storage - ✔✔1-6C
,washed with sterile normal saline to remove most of plasma
24 hour expiration
✔✔FFP storage and description - ✔✔prepared from WB or collected using apheresis.
frozen at -18C or below
contains max levels of labile or nonlabile coag factors
Thaw between 30-37C
✔✔Thawed plasma - ✔✔FFP thawed but not transfused within 24 hrs
can be stored 1-6C for 5 days
reduction in Factors V and VII
✔✔Liquid plasma - ✔✔plasma separated from WB at any time during storage, up to 5
days after the expiration date of WB. supernatant levels of potassium and ammonia are
increased
✔✔Cryo - ✔✔prepared from WB collected into CPDA-1 or CPD
precipitates when FFP thawed between 1-6C
stored at -18C or colder
suspended in less than 15mL plasma
contains factor VII and fibrinogen and most of XIII
single unit store at 20-24 for 6 hrs
pooled store at 20-24 for 4 hours
✔✔platelets - ✔✔prepared from WB that hasn't cooled below 20C
separated within 4 hours
soft spin (plt rich plasma) followed by a hard spin (platelet concentrate)
resuspended in about 45-65mL plasma
20-24C with gentle agitation
✔✔Granulocytes - ✔✔usually harvested in buffy coats
collection must yield 1.0x10 to the 10th granulocytes
Transfuse ASAP
✔✔Irradiation - ✔✔prevents proliferation of transfused T-Lymphs, the primary cause of
graft vs host (GVHD)
✔✔effects of storage on blood components - ✔✔decreases 2,3-DPG levels: this
controls release of O2 from RBCs, levels recover after 24 hrs post txn
decreases ATP
increases potassium
✔✔Leukocyte reduction must reduce WBC count to what? - ✔✔<5x10 to the sixth in at
least 95% of units samples and yield >85% RBC recovery
, ✔✔What must the minimum mean volume of Apheresis RBCs be? - ✔✔180mL packed
RBC volume per unit (>60g hgb)
95% of samples units must have at least 150mL (>50g hgb)
✔✔What must the minimum mean volume of Apheresis RBCs leukoreduced be? -
✔✔<5x10 to the sixth WBC and mean volume of 153mL packed RBC volume per unit
(>51g Hgb)
95% of sample units have at least 128mL (>42.5g hgb)
✔✔How much coag factor VIII and fibrinogen must cryo have? - ✔✔80IU Factor VIII and
150mg fibrinogen
pooled units must have same amt multipled by number of units in pool
✔✔What are Mendel's principles? - ✔✔random segregation: distinct genes are
randomly inherited one from each parent
✔✔what is independent assortmant (Mendel's principle)? - ✔✔genes inherited
independently if carried on different chromosomes.
combos of genes are not dependent on other genes (exception: linkage)
✔✔what is an autosome? - ✔✔any chromosome other than the sex chromosomes
✔✔What is meiosis? - ✔✔process by which sex cells are produced which have only one
copy of each chromosome (haploid).
✔✔What is mitosis? - ✔✔process where sex cells are duplicated and equally divided
between two daughter cells
✔✔zygote - ✔✔fertilized egg
✔✔gamete - ✔✔sex cells....egg and sperm
✔✔diploid - ✔✔normal number of chromosomes in zygote and all body cells (46)
✔✔haploid - ✔✔normal number of chromosomes in all gametes (23)
✔✔structural gene - ✔✔gene that controls the protein production of another gene or
genes
✔✔regulator gene - ✔✔gene that controls the rated of production of another gene or
genes
✔✔allele - ✔✔alternate forms of a gene at a given locus
