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Technologist in Molecular Biology, MB(ASCP) board exam prep 2024 with 100% Correct Answers
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Technologist in Molecular Biology, MB(ASCP) 
board exam prep 2024 with 100% Correct 
Answers 
Pyrimidine - Answer ️️ -One carbon ring 
Cytosine, Thymine, Uracil 
What is the function of mRNA? - Answer ️️ -Carries genetic info out of nucleus 
Transcript translated to protein 
What is the function of tRNA? - Answer ️️ -Carries aa to ribosome 
Anticodon pairs with codon on mRNA strand 
What is the function of rRNA? - Answer ️️ -part of ribosome structure 
most abundant RNA 
coordina...
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ASCP Molecular Biology Certification Exam 2024
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ASCP Molecular Biology Certification Exam 2024 
Pyrimidine - Answer ️️ -One carbon ring 
Cytosine, Thymine, Uracil 
Purine - Answer ️️ -Two carbon rings 
Adenine, Guanine 
How are nucleotides joined together? - Answer ️️ -Condensation to form phosphodiester bond 
What is the function of mRNA? - Answer ️️ -Carries genetic info out of nucleus 
Transcript translated to protein 
What is the function of tRNA? - Answer ️️ -Carries aa to ribosome 
Anticodon pairs with codon on mRNA ...
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MB (ASCP) 2024 - Translocations, Diseases, Pharma, Polymerases, & more
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MB (ASCP) 2024 - Translocations, Diseases, 
Pharma, Polymerases, & more 
Chronic Myeloid Leukemia (CML) - Answer ️️ -t(9;22) 
BCR-ABL translocation 
Ewing sarcoma - Answer ️️ -t(11;22) 
EWS-FLI1 
Acute Lymphocytic Leukemia (ALL) - Answer ️️ -t(9;22) 
BCR-ABL 
p190 protein 
Follicular Lymphoma - Answer ️️ -t(14;18) 
BCL2 translocation 
Mantle Cell Lymphoma - Answer ️️ -t(11;14) bcl-1 translocation 
CCND1-IgH 
Burkitt Lymphoma - Answer ️️ -t(8;14) 
MYC gene translocation 
R...
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MB (ASCP) Practice Exam 2024 100% Correct
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MB (ASCP) Practice Exam 2024 100% Correct 
What does barcoding do in Next Generation Sequencing? - Answer ️️ -Adds unique tag to all 
nucleic acids within one sample so that various samples can be mixed 
What is the best way to store RNA for longer than a month? - Answer ️️ -In Ethanol, at -20°C 
or -80°C 
Testing for Aldehyde Dehydrogenase deficiency is important in patients with: - Answer ️️ - 
Alcohol dependence 
During DNA replication, the 3' -OH of the growing DNA chain attac...
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MB(ASCP) Exam Practice Latest Update 2024
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MB(ASCP) Exam Practice Latest Update 2024 
what does topoisomerase do? - Answer ️️ -breaks phosphate backbone to "un" 
supercoil DNA 
Another name for Topoisomerase II - Answer ️️ -Gyrase 
Helicase - Answer ️️ -Un"zips" DNA at replication fork 
What makes up the primosome? - Answer ️️ -1. primase 
2. helicase 
3. DNA C 
NASBA stands for... - Answer ️️ -nucleic acid sequence based amplification 
NASBA - Answer ️️ -RNA is target 
RNA-->cDNA 
Reverse transcriptase (RN...
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ASCP MB Exam Questions and Answers 100% Pass
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ASCP MB Exam Questions and Answers 100% Pass 
Acute Promylocity Leukemia (APL) - Answer ️️ -t(15:17) 
PML / RARa genes 
Chronic Myelogenous Leukemia (CML) - Answer ️️ -t(9:22) 
a.k.a. Philadelphia chromosome 
ABL1 / BCR genes 
Burkitt's non-Hodkin's Lymphoma - Answer ️️ -t(8:14) 
dysregulation of c-myc gene 
breakpoints on ch.8 spread over 190kbp region 5' to 3' 
Follicular non-Hodkin's Lymphoma - Answer ️️ -t(14:18) 
IgH / BCL2 genes 
Mantle Cell Lymphoma (MCL) - Answer ️...
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MB ASCP 2024 Exam Grade A
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MB ASCP 2024 Exam Grade A 
What genes would be screened in a lung cancer panel? - Answer ️️ -KRAS, EGFR, ALK 
In a retrovirus, the RNA acts as: - Answer ️️ -Genome and mRNA 
To what does the AcrAB gene product create resistance to? - Answer ️️ -Tetracycline 
What gene is measured following the treatment with imatinib(Gleevec)? - Answer ️️ - 
BCR/Abl 
Blood drawn into what type of tube is compatible for genetic testing? - Answer ️️ -K2-EDTA 
tube 
Primer dimers are due to what...
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MB (ASCP) Exam Questions and Answers Graded A
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MB (ASCP) Exam Questions and Answers Graded A 
Predominant form of DNA - Answer ️️ -Right-handed B 
DNA and histone interaction is due to what type of bond? - Answer ️️ -Ionic 
What stains to the minor groove of DNA duplex? - Answer ️️ -SYBR Green 
What is the most abundant type of RNA? - Answer ️️ -Ribosomal RNA (rRNA) 
What does RNA act as in a retrovirus? - Answer ️️ -Genome and mRNA 
What increases half-life of mRNA? - Answer ️️ -5' Methyl Cap 
What kind of DNA is mo...
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Chapter 4 - Nucleic Acid Extraction Question and Answer 100% Correct
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Chapter 4 - Nucleic Acid Extraction 
Question and Answer 100% Correct 
Organic - DNA extraction method; uses organic chemicals, phenol and 
chloroform 
What substances are best used to store tissue for excellent DNA recovery? 
- Acetone; 10% buffered neutral formalin 
Phenol - Degrades proteins; used in organic extraction method of DNA 
Chloroform - Removes phenol; used in organic extraction method of 
DNA 
EDTA - Ethylenediamine Tetra-acetic Acid; Chelating agent, binds 
metal ions to prevent D...
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Bonus Question and Answer 100% Correct
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Bonus Question and Answer 100% 
Correct 
polymerase slippage - DNA polymerase can lose its place in a repetitive 
sequence tract, and either leaves out or add an inappropriate number of 
repeat units 
Imatinib - tyrosine kinase inhibitor (Gleevec) 
What is the rate of mutation per round of DNA replication? 
A. 1 in 1,000 base pairs 
B. 1 in 10,000 base pairs 
C. 1 in 1,000,000 base pairs 
D. 1 in 1,000,000,000 base pairs - D. 1 in 1,000,000,000 base pairs 
Which of the following is not a probe-l...
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