Brainarium

Antibody → Gene - ️️Immunoprecipitation coupled with mass spec & computer search Antibody → Protein expression, localization, & dynamics - ️️Immunofluorescence microscopy, western blot Transcription Profile → Gene - ️️RNA-seq Gene → Transcription Profile - ️️Northern blot, GFP reporter

what else is needed for transcription in vivo besides the promoter? - ️️the enhancer > used deletion and linker scanner analysis to further define enhancer properties of enhancers - ️️-100-200bp -densely packed with consensus sequences -enhancers act in a distance and orientation dependent fashion -work with virtually any promoter -mediate regulation identifying proteins that regulate transcription - ️️-RNA polymerases purified based on "random" RNA synthesis cell e...

Which of the following is the same as a continuous trait? - ️️Quantitative and Polygenic trait Which of the following can contribute to the continuous nature of continuous traits? (Select 'True' or 'False' for each) - ️️True: Many alleles of individual genes within the population True: Multiple genes contributing to a single phenotype True: Multiple pathways contributing to a single phenotype True: Dosage effects How many alleles are contained in the list below? trp1...

Why do you need a selectable marker on a bacterial plasmid? - ️️-transformation efficiency is low -need to identify which subset of cells took up the plasmid Wild-type yeast strains are: - ️️prototrophic Wild-type yeast strains grown on: - ️️-rich media -minimal media The majority of yeast strains that are useful in the laboratory are: - ️️auxotrophic

The consensus elements required for transcription of tRNA and 5sRNA genes by RNAPIII: - ️️Correct: are found within the tRNA and 5S RNA coding regions. What is the general name for the type of DNA elements that allow eukaryotic genes to be turned on under certain specific conditions, such as in the presence of glucocorticoid hormones? - ️️Correct: response element Which eukaryotic control region typically mediates regulation of transcription? - ️️Enhancer As discussed in lectu...

What is multiplex PCR? - ️️- a PCR reaction that uses a single source of sample DNA and more than one primer set. In a standard deck of playing cards, there are four suits (red suits = hearts and diamonds, black suits = spades and clubs). Each suit has thirteen cards: Ace, 2, 3, 4, 5, 6, 7, 8, 9, 10, and the face cards Jack, Queen and King. In a single draw, what is the probability that you will draw: A red card? - ️️1/2 In a standard deck of playing cards, there are four suits (r...

Based on our discussion in lecture, expression of the eyeless gene in Drosophila is: - ️️Correct: both necessary and sufficient to initiate the process of eye development. Assignment 1 » 5 (2 pts) You clone the wild-type version of a gene and engineer it to be ectopically expressed. Based on our discussion in lecture, is your newly constructed gene more accurately described as a - ️️Correct: Gain-of-function allele. Genetics can be used to refer either to a scientific discipline o...

What is the DNA content of a human cell in meiosis at the time of recombination but prior to the first meiotic division? - ️️4n ASO hybridization is used to detect which type of polymorphism? - ️️SNP Which of the following techniques could be used to genotype the alleles at a locus that contains a microsatellite? - ️️-Southern blot -PCR As described in lecture, the term Genetics can be used to refer to either the scientific discipline of inheritance or to a set of technologies...

Make two primers specific to genes, on one end add a sequence that corresponds to one restriction enzyme(HIND), and the other a different restriction enzyme (BAM) to the other end and amplify linker scanner mutagenisis - ️️a systemic way to determine 10bp necessary for txn Boxes indicate a place where we've lost transcription, so we need that DNA. if we lost transcription with 3 substitutions, our promoter needs those 3 elements construction of linker scanner mutant - ️️confusin...

What does a 1:1:4 ratio of PD:NPD:T tell us? - ️️It indicates that two genes are assorting randomly What does PD>NPD tell us? - ️️It indicates that the two genes are linked What does a 1:1<4 ratio of PD:NPD:T tell us? - ️️It indicates that each of the two genes are linked to different centromeres What happens when genes are close (linked) to a centromere? - ️️The frequency of recombination is drastically reduced When 2 genes are linked what is the most common event? ...

T/F The ovule is a series of nested structures that provides protection for the female gametophyte and embryo if it is fertilized - ️️True T/F Pollen increased the need for water to be present for fertilization to occur in land plants - ️️False T/F Tracheophytes have dependent sporophytes - ️️False gametophytes grow on sporophytes T/F Often in plants, the male gametophyte provides some sort of nourishment to the developing embryo called matrotrophy - ️️False the femal...

What is Interval level data? - ️️continuous actual numbers with no real 0 There is an assumption that there are equal intervals between points on a scale Two classic interval scales are Likert and the semantic scale Ratio - ️️continuous numbers with a REAL 0 There are actual zeros in comm. Class attendance going to the movies, zero income, zero employment, zero formal education if you've never attended school Central Tendency - ️️3 basic statistics used to summarize data are...

Natural Hazard - ️️Natural process which poses a threat to human life or property Example: Stream Flood Natural Disaster - ️️Natural event that causes significant damage to life or property Example: Snowmageddon (U.S. Feb. 2010, largest snow storm in 90 years, some areas saw 24+ inches of snow) Natural Catastrophe - ️️Natural event that kills or injures large numbers of people or causes major property damage Example: Haiti Earthquake (Jan. 2010, Magitude 7, 230,000 killed, 300...

Who is a respondeat superior? - ️️An employer is liable for the careless acts of an employee (negligent acts) of the employees which occur within the scope of employment Doctrine stands for "make the master accountable" in Latin What type of law is practiced in an employer - employee relationship? - ️️Tort law Ex: respondeat superior Ex: Hanson's furniture store: from Hank to the customer. What is person - independent contractor relationship? - ️️The "agent" is ...

4 main functions of managers - ️️planning organizing leading controlling 3 main managerial roles - ️️informational interpersonal decisional interpersonal - ️️1. Figurehead Role 2. Leader Role 3. Liaison Role informational - ️️1. Monitoring Role 2. Disseminator Role 3. Spokesman Role efficiency - ️️allocating resources correctly with no waste

Demands - ️️backed by buying power Market Offerings - ️️some combination of products, services, information, or experiences offered to a market to satisfy a need or want Marketing Myopia - ️️is focusing only on existing wants and losing sight of underlying consumer needs Market or Market Segmentation - ️️is a set of actual and potential buyers (Suppliers, Company, Competitors, Marketing Intermediaries, Final Consumers) Marketing Management - ️️the art and science of ch...

Evolution - ️️What is a unifying concept in biology that explains the diversity of life? Genome - ️️The ___________, which refers to the complete genetic composition of an organism, provides continuity from one generation to the next. DNA - ️️Genomics involves the analysis of the ____________ sequences of genomes. Proteins - ️️The role of most genes is to encode mRNAs that are used in the production of ____________. Prokaryotic ; Eukaryotic - ️️____________ organisms d...

What are Content Results? - ️️"getting your idea across" - enabling other to know/understand what you want them to know/understand - the completion of the process of constructing meaning What are Relationship Results? - ️️often overlooked - human connection communications contract when managing mutual responding What is Phronimos? - ️️takes appropriate action in the right way, effectively balancing good and expedient What is Tikkun Olam? - ️️contributing to the repai...

Disadvantages of corporations? - ️️-separation of ownership and management (agency problem, sometime the owners do what's best for them and not for the stockholder) -Double taxation meaning corporation report their income statement, gets to their taxable income, pays taxes on that, gets to their net income and decides they can either pay it out in dividends or reinvest it back into the company but if they pay it out in dividends, those dividends are taxed again on the personal level...

which of the following activities is not included in on duty time - Correct Answer-resting in sleeper Who should investigate at scene - Correct Answer-person designated to control work place hazards, first step - Correct Answer-engineering controls or eliminate by design carriers may allow new driver to drive while waiting on results from MRO - Correct Answer-False effectiveness of safety meeting - Correct Answer-all of the above A vehicle will be flagged every time if it is at - Correct...