Fixation

First and most critical step







Primary purpose of Fixation is:

preserve the morphological and chemical integrity of the cell







Secondary purpose of Fixation is:

harden and protect the tissue , thus easier to cut







Size of tissue to be processed

ideal size is 3mm







Amount of fixative

10-20 times volume of specimen (except Osmium tetroxide)







Ideal ratio of tissue:fixative

1:10







Hydrogen ion concentration

pH 6.0-8.0







Temperature for routine

RT
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