Kcat Study guides, Class notes & Summaries

Chem and Physics section - correct answer What atom is the site of covalent attachment of AMC to the model tetrapeptide used in the studies? - correct answer Because AMC is attached to the peptide on the carboxyl side. This suggests that an amide linkage involving the N atom in AMC is used to covalently attach the fluorophore to the peptide *a peptide bond between amino acids= covalent bond between carboxyl group of 1 amino acid to amino group of other, releasing water What express...

What is kcat? What is a holoenzyme? Correct Answer-Kcat=overall rate of an enzyme, catalytic efficiencies. Holoenzyme (active) is an inactive apoenzyme with non-protein components. (look at the picture) Discuss enzyme-substrate specificity. What is the lock and key vs. induced fit model? Correct Answer-Induced fit: enzyme changes after substrate binds to make it fit more perfectly. Discuss energy implications of enzymes. Does it change the activation energy or free energy of the overal...

AAMC MCAT Practice Exam 2 Questions and Answers 2024 C/P: What expression gives the amount of light energy (in J per photon) that is converted to other forms between the fluorescence excitation and emission events? "intensity of fluorescence emission at 440 nm excitation at 360 nm) was monitored for 20 minutes" A) (6.62 × 10-34) × (3.0 × 108) B) (6.62 × 10-34) × (3.0 × 108) × (360 × 10-9) C) (6.62 × 10-34) × (3.0 × 108) × [1 / (360 × 10-9) - 1 / (440 × 10-9)] D) (6.62 × ...

FL 5 - C/PWhat quantity of Compound 1 must be provided to prepare 100.00 mL of solution with a concentration equal to Ki? A.48.4 mg B.24.2 mg C.5.64 mg D.2.92 mg - correct answer D.2.92 mg - 1. find moles - n = c (molarity) x litres - 2. find mass - m = moles x molar mass - watch units If only [I] is increased, then [ESI] or [EI] increases. This is an example of: A.the Bose-Einstein Principle. B.the Heisenberg Uncertainty Principle. C.the Le Châtelier's Principle. D.the Pauli Exc...

BCH 400: Exam Questions & Answers 2023/2024 Enzyme cofactors that bind covalently at the active site of an enzyme are referred to as _________. - ANSWER-prosthetic groups. Which of the following kinetic parameters best describes how well suited a specific compound functions as a substrate for a particular enzyme? - ANSWER-kcat/Km Which of the following is characteristic of an enzyme catalyst? - ANSWER-It positions reactants in the correct orientation. It lowers the activation energy ba...

AAMC MCAT Practice Exam 2 Questions and Answers 2024 C/P: What expression gives the amount of light energy (in J per photon) that is converted to other forms between the fluorescence excitation and emission events? "intensity of fluorescence emission at 440 nm excitation at 360 nm) was monitored for 20 minutes" A) (6.62 × 10-34) × (3.0 × 108) B) (6.62 × 10-34) × (3.0 × 108) × (360 × 10-9) C) (6.62 × 10-34) × (3.0 × 108) × [1 / (360 × 10-9) - 1 / (440 × 10-9)] D) (6.62 × ...

UKCAT quantitative reasoning

AAMC MCAT PRACTICE EXAM 2 | QUESTIONS & ANSWERS| ALREADY PASSED| VERIFIED & UPDATED C/P: What expression gives the amount of light energy (in J per photon) that is converted to other forms between the fluorescence excitation and emission events? "intensity of fluorescence emission at 440 nm excitation at 360 nm) was monitored for 20 minutes" A) (6.62 × 10-34) × (3.0 × 108) B) (6.62 × 10-34) × (3.0 × 108) × (360 × 10-9) C) (6.62 × 10-34) × (3.0 × 108) × [1 / (360 × 10-9) -...

AAMC MCAT PRACTICE EXAM 2 QUESTIONS AND ANSWERS RATED A+ 2023|2024 C/P: What expression gives the amount of light energy (in J per photon) that is converted to other forms between the fluorescence excitation and emission events? "intensity of fluorescence emission at 440 nm excitation at 360 nm) was monitored for 20 minutes" A) (6.62 × 10-34) × (3.0 × 108) B) (6.62 × 10-34) × (3.0 × 108) × (360 × 10-9) C) (6.62 × 10-34) × (3.0 × 108) × [1 / (360 × 10-9) - 1 / (440 × 10-9)...

1. A weak acid, HA, has a pKa of 4.11. a) At pH 7.11, what will the ratio of conjugate base to acid be? (Henderson-Hasselbach equation: pH=pKa + log [A-]/[HA]) b) Based on answer to "a," is there more HA or A- at that pH? c) At pH 2.11, what will the ratio of conjugate base to acid be? d) Based on answer to "c," is there more HA or A- at that pH? e) At pH 4.11, what will the ratio of conjugate base to acid be? f) Fill in the blank: if pH is greater than the pKa of a weak acid, there w...