QPCR Study guides, Class notes & Summaries

ASCP MB: Extra-Practice Questions and Answers Scored A The Klenow fragment of E. coli DNA polymerase I is used: to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucleic acid interaction. ...

1 / 14 1. PCR can test for what diseases?: Hep - B Herpes simplex 1 Herpes simplex 2 HIV HPV Epstein-barr Cytomegalovirus Cystic fibrosis Huntingtons Bk virus Fragile X Parovirus 2. qPCR can test for what diseases?: Herpes simplex 1 Herpes simplex 2 Epstein-barr HPV HIV Cytomegalovirus Bk virus VZV 3. RT PCR can test for what diseases?: HIV Influenza Coronavirus Ewing sarcoma Synovial sarcoma Hep - C 4. Multiplex PCR can test for what diseases?: B. pertussis M. pneumo...

MB ASCP QUESTIONS AND ANSWERS SCORED A+ What genes would be screened in a lung cancer panel? KRAS, EGFR, ALK In a retrovirus, the RNA acts as: Genome and mRNA To what does the AcrAB gene product create resistance to? Tetracycline What gene is measured following the treatment with imatinib(Gleevec)? BCR/Abl Blood drawn into what type of tube is compatible for genetic testing? K2-EDTA tube Primer dimers are due to what complimentary issue? 3' to 3' complementarity of primer pairs Microsate...

MB (ASCP) 2024 - Translocations, Diseases, Pharma, Polymerases, & more Chronic Myeloid Leukemia (CML) - Answer ️️ -t(9;22) BCR-ABL translocation Ewing sarcoma - Answer ️️ -t(11;22) EWS-FLI1 Acute Lymphocytic Leukemia (ALL) - Answer ️️ -t(9;22) BCR-ABL p190 protein Follicular Lymphoma - Answer ️️ -t(14;18) BCL2 translocation Mantle Cell Lymphoma - Answer ️️ -t(11;14) bcl-1 translocation CCND1-IgH Burkitt Lymphoma - Answer ️️ -t(8;14) MYC gene translocation R...

What genes would be screened in a lung cancer panel? - correct answer KRAS, EGFR, ALK In a retrovirus, the RNA acts as: - correct answer Genome and mRNA To what does the AcrAB gene product create resistance to? - correct answer Tetracycline What gene is measured following the treatment with imatinib(Gleevec)? - correct answer ...

This DNA amplification method amplifies the nucleic acid used as a probe? - ANSWER Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? - ANSWER DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? - ANSWER Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? - ANSWER Better specificity How does Ligase Chain Reaction differ from Polymerase Chain ...

GENE 4200 practice exam 1|26 Questions with Verified Answers Transcription factors help regulate which cell types and tissues will express a given gene (T/F) - CORRECT ANSWER When comparing RNA levels for gene X between two samples by qPCR, the sample with thehighest DCT value would have the least gene X RNA (T/F) - CORRECT ANSWER Exons are the most abundant DNA sequences in the human genome (T/F) - CORRECT ANSWER false Most transposons in the human genome are inactive. (T/F) - COR...

MB (ASCP) Exam Questions and Answers Graded A+ Predominant form of DNA Right-handed B DNA and histone interaction is due to what type of bond? Ionic What stains to the minor groove of DNA duplex? SYBR Green What is the most abundant type of RNA? Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? Genome and mRNA What increases half-life of mRNA? 5' Methyl Cap What kind of DNA is most likely to be transcriptionally active? Euchromatin What is the most common type of eukaryot...

MB (ASCP) Practice Exam Questions and Answers 2023 What does barcoding do in Next Generation Sequencing? Adds unique tag to all nucleic acids within one sample so that various samples can be mixed What is the best way to store RNA for longer than a month? In Ethanol, at -20°C or -80°C Testing for Aldehyde Dehydrogenase deficiency is important in patients with: Alcohol dependence During DNA replication, the 3' -OH of the growing DNA chain attacks which phosphate of an ...

(MB) ASCP Practice Exam Questions and Answers Solved Correctly Which of the following is not a component of a nucleotide? Phosphate group Anti-codon Ribose sugar Nitrogen base Anti-codon According to Chargaff's rule of base pairing, adenine pairs with: Thymine What genes would be screened in a breast cancer panel? HER2, ERBB2, BRCA1 Next Generation Sequencing uses: Short sequence reads Purines and pyrimidines differ from each other in that: Purines have two rings; pyrimidines have one r...