QPCR Study guides, Class notes & Summaries

AAB Molecular Diagnostics Testing with complete solutions 2023This DNA amplification method amplifies the nucleic acid used as a probe? Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? Better specificity How does L...

MB (ASCP) Review & Exam Questions Part II Questions and answers 2023 What is the Beer-Lambert Law? the linear relationship between absorbance and concentration of an absorbing species. What is the Beer-Lambert Law equation? A = ebc A: absorbance e: molar absorptivity c: concentration b: path length DNA and RNA are absorbed at: 260nm Proteins are absorbed at: 280nm What is the melting temperature equation? Tm = (4 x # of GC pairs) + (2 x # of AT p...

1 / 14 1. PCR can test for what diseases?: Hep - B Herpes simplex 1 Herpes simplex 2 HIV HPV Epstein-barr Cytomegalovirus Cystic fibrosis Huntingtons Bk virus Fragile X Parovirus 2. qPCR can test for what diseases?: Herpes simplex 1 Herpes simplex 2 Epstein-barr HPV HIV Cytomegalovirus Bk virus VZV 3. RT PCR can test for what diseases?: HIV Influenza Coronavirus Ewing sarcoma Synovial sarcoma Hep - C 4. Multiplex PCR can test for what diseases?: B. pertussis M. pneumo...

MB (ASCP) 2022 - Translocations, Diseases, Pharma, Polymerases, & more Chronic Myeloid Leukemia (CML) t(9;22) BCR-ABL translocation Ewing sarcoma t(11;22) EWS-FLI1 Acute Lymphocytic Leukemia (ALL) t(9;22) BCR-ABL p190 protein Follicular Lymphoma t(14;18) BCL2 translocation Mantle Cell Lymphoma t(11;14) bcl-1 translocation CCND1-IgH Burkitt Lymphoma t(8;14) MYC gene translocation Rhabdomyosarcoma (alveolar); Cancer/solid tumor t(2;13) or t(1;13) PAX3-FKHR and PAX7-FKHR "ARMS" translocatio...

(MB) ASCP Practice Exam Questions Answered 100% correct Which of the following is not a component of a nucleotide? Phosphate group Anti-codon Ribose sugar Nitrogen base Anti-codon According to Chargaff's rule of base pairing, adenine pairs with: Thymine What genes would be screened in a breast cancer panel? HER2, ERBB2, BRCA1 Next Generation Sequencing uses: Short sequence reads Purines and pyrimidines differ from each other in that: Purines have two rings...

Which of the following is not a component of a nucleotide? Phosphate group Anti-codon Ribose sugar Nitrogen base Anti-codon According to Chargaff's rule of base pairing, adenine pairs with: Thymine What genes would be screened in a breast cancer panel? HER2, ERBB2, BRCA1 Next Generation Sequencing uses: Short sequence reads Purines and pyrimidines differ from each other in that: Purines have two rings; pyrimidines have one ring The purines are: Cytosine and uracil Adenine and thymin...

MB (ASCP) 2024 - Translocations, Diseases, Pharma, Polymerases, & more Chronic Myeloid Leukemia (CML) - Answer ️️ -t(9;22) BCR-ABL translocation Ewing sarcoma - Answer ️️ -t(11;22) EWS-FLI1 Acute Lymphocytic Leukemia (ALL) - Answer ️️ -t(9;22) BCR-ABL p190 protein Follicular Lymphoma - Answer ️️ -t(14;18) BCL2 translocation Mantle Cell Lymphoma - Answer ️️ -t(11;14) bcl-1 translocation CCND1-IgH Burkitt Lymphoma - Answer ️️ -t(8;14) MYC gene translocation R...

GENE 4200 practice exam 1|26 Questions with Verified Answers Transcription factors help regulate which cell types and tissues will express a given gene (T/F) - CORRECT ANSWER When comparing RNA levels for gene X between two samples by qPCR, the sample with thehighest DCT value would have the least gene X RNA (T/F) - CORRECT ANSWER Exons are the most abundant DNA sequences in the human genome (T/F) - CORRECT ANSWER false Most transposons in the human genome are inactive. (T/F) - COR...

This DNA amplification method amplifies the nucleic acid used as a probe? - ANSWER Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? - ANSWER DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? - ANSWER Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? - ANSWER Better specificity How does Ligase Chain Reaction differ from Polymerase Chain ...

1 / 14 1. PCR can test for what diseases?: Hep - B Herpes simplex 1 Herpes simplex 2 HIV HPV Epstein-barr Cytomegalovirus Cystic fibrosis Huntingtons Bk virus Fragile X Parovirus 2. qPCR can test for what diseases?: Herpes simplex 1 Herpes simplex 2 Epstein-barr HPV HIV Cytomegalovirus Bk virus VZV 3. RT PCR can test for what diseases?: HIV Influenza Coronavirus Ewing sarcoma Synovial sarcoma Hep - C 4. Multiplex PCR can test for what diseases?: B. pertussis M. pneumo...