QPCR Study guides, Class notes & Summaries

AAB Molecular Diagnostics Testing with complete solutions 2023This DNA amplification method amplifies the nucleic acid used as a probe? Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? Better specificity How does L...

MB (ASCP) 2022 - Translocations, Diseases, Pharma, Polymerases, & more Chronic Myeloid Leukemia (CML) t(9;22) BCR-ABL translocation Ewing sarcoma t(11;22) EWS-FLI1 Acute Lymphocytic Leukemia (ALL) t(9;22) BCR-ABL p190 protein Follicular Lymphoma t(14;18) BCL2 translocation Mantle Cell Lymphoma t(11;14) bcl-1 translocation CCND1-IgH Burkitt Lymphoma t(8;14) MYC gene translocation Rhabdomyosarcoma (alveolar); Cancer/solid tumor t(2;13) or t(1;13) PAX3-FKHR and PAX7-FKHR "ARMS" translocatio...

1 / 14 1. PCR can test for what diseases?: Hep - B Herpes simplex 1 Herpes simplex 2 HIV HPV Epstein-barr Cytomegalovirus Cystic fibrosis Huntingtons Bk virus Fragile X Parovirus 2. qPCR can test for what diseases?: Herpes simplex 1 Herpes simplex 2 Epstein-barr HPV HIV Cytomegalovirus Bk virus VZV 3. RT PCR can test for what diseases?: HIV Influenza Coronavirus Ewing sarcoma Synovial sarcoma Hep - C 4. Multiplex PCR can test for what diseases?: B. pertussis M. pneumo...

MB (ASCP) Review & Exam Questions Part II Questions and answers 2023 What is the Beer-Lambert Law? the linear relationship between absorbance and concentration of an absorbing species. What is the Beer-Lambert Law equation? A = ebc A: absorbance e: molar absorptivity c: concentration b: path length DNA and RNA are absorbed at: 260nm Proteins are absorbed at: 280nm What is the melting temperature equation? Tm = (4 x # of GC pairs) + (2 x # of AT p...

(MB) ASCP Practice Exam Questions Answered 100% correct Which of the following is not a component of a nucleotide? Phosphate group Anti-codon Ribose sugar Nitrogen base Anti-codon According to Chargaff's rule of base pairing, adenine pairs with: Thymine What genes would be screened in a breast cancer panel? HER2, ERBB2, BRCA1 Next Generation Sequencing uses: Short sequence reads Purines and pyrimidines differ from each other in that: Purines have two rings...

Which of the following is not a component of a nucleotide? Phosphate group Anti-codon Ribose sugar Nitrogen base Anti-codon According to Chargaff's rule of base pairing, adenine pairs with: Thymine What genes would be screened in a breast cancer panel? HER2, ERBB2, BRCA1 Next Generation Sequencing uses: Short sequence reads Purines and pyrimidines differ from each other in that: Purines have two rings; pyrimidines have one ring The purines are: Cytosine and uracil Adenine and thymin...

ASCP MB: Extra-Practice Questions and Answers Scored A The Klenow fragment of E. coli DNA polymerase I is used: to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucleic acid interaction. ...

1 / 14 1. PCR can test for what diseases?: Hep - B Herpes simplex 1 Herpes simplex 2 HIV HPV Epstein-barr Cytomegalovirus Cystic fibrosis Huntingtons Bk virus Fragile X Parovirus 2. qPCR can test for what diseases?: Herpes simplex 1 Herpes simplex 2 Epstein-barr HPV HIV Cytomegalovirus Bk virus VZV 3. RT PCR can test for what diseases?: HIV Influenza Coronavirus Ewing sarcoma Synovial sarcoma Hep - C 4. Multiplex PCR can test for what diseases?: B. pertussis M. pneumo...

MB (ASCP) 2024 - Translocations, Diseases, Pharma, Polymerases, & more Chronic Myeloid Leukemia (CML) - Answer ️️ -t(9;22) BCR-ABL translocation Ewing sarcoma - Answer ️️ -t(11;22) EWS-FLI1 Acute Lymphocytic Leukemia (ALL) - Answer ️️ -t(9;22) BCR-ABL p190 protein Follicular Lymphoma - Answer ️️ -t(14;18) BCL2 translocation Mantle Cell Lymphoma - Answer ️️ -t(11;14) bcl-1 translocation CCND1-IgH Burkitt Lymphoma - Answer ️️ -t(8;14) MYC gene translocation R...

MB ASCP QUESTIONS AND ANSWERS SCORED A+ What genes would be screened in a lung cancer panel? KRAS, EGFR, ALK In a retrovirus, the RNA acts as: Genome and mRNA To what does the AcrAB gene product create resistance to? Tetracycline What gene is measured following the treatment with imatinib(Gleevec)? BCR/Abl Blood drawn into what type of tube is compatible for genetic testing? K2-EDTA tube Primer dimers are due to what complimentary issue? 3' to 3' complementarity of primer pairs Microsate...