- Study guides, Class notes & Summaries

On which of the plates would you expect to find bacteria most like the original untransformed E. coli colonies you initially observed? Explain your prediction. - Answer Bacteria which resemble the non-transformed will be found on the LB/(-) pGLO plate. These bacteria were removed from the starter plate, did not have any plasmid added to them, and were replated on an LB plate. Thus, they are virtually identical to the non-transformed starter. If there are any genetically transformed bacteri...

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3 pages

Written in 2024/2025

Grade A+

TestSolver9

To genetically transform an entire organism, you must insert the new gene into every cell in the organism. Which organism is better suited for total genetic transformation- one composed of many cells, or one composed of a single cell? - Answer A single-celled organism, because it contains only one cell to take up the new gene. Scientists often want to know if the genetically transformed organism can pass its new traits on to its offspring and future generations. To get this information, wh...

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3 pages

Written in 2024/2025

Grade A+

TestSolver9

what was function of incubation on ice - ANSWER -Incubation slows down the movement of the bilayer in cell. slows fluid cell membrane- fluid mosatic model what was the function of adding calcluim chloride - ANSWER -Addition of calcium chloride solution- the calcium has a positive charger so it shields the negative phosphate in the nucliotide. You nutrelize it so it can go through the cell membrane because it is non polar. Adding the calcium chloride serves to shield the negative charg...

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5 pages

Written in 2025/2026

Grade A+

MERCYTRISHIA

To genetically transform an entire organism, you must insert the new gene(s) into every cell in the organism. Which organism is better suited for total genetic transformation-one composed of many cells, or one composed of a single cell? - Answer A single-celled organism would be the best recipient for a genetic transformation, because it contains only one cell which needs to take up the new gene. Scientists often want to know if the genetically transformed organism can pass its new traits ...

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15 pages

Written in 2024/2025

Grade A+

TestSolver9

the ability of the cells to live and reproduce in the presence of an antibiotic such as ampicillin - Answer the cells cannot survive, so they'll die describe how you could use two LB/agar plates, some e. coli, and some ampicillin to determine how e. coli cells are affected by ampicillin - Answer - you have to put equal amounts of e. coli on the two LB/agar plates - one will contain agar and the other will contain ampicillin - if there are fewer cells left than there originally were...

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3 pages

Written in 2024/2025

Grade A+

TestSolver9

transformation - Answer a change in phenotype or genotype characterized by the assimilation of external DNA by a cell gel electrophoresis - Answer technique used to separate DNA fragments according to their size and charge; involved running a current through a gel containing molecules of interest Off, inducible - Answer The lac operon is _____ by default, making it an _____ operon. Transformation in pGlo - Answer mixing the GFP (green fluorescent protein) LB plate - Answe...

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3 pages

Written in 2024/2025

Grade A+

TestSolver9

Calcium chloride/heat shock - Answer method of transformation bacterial transformation - Answer Taking up of DNA from the environment by bacterial cells plasmid - Answer a DNA molecule that is usually found in bacteria and that can replicate independent of the main chromosome bla gene - Answer ampicillin resistant gene, beta-lactamase enzyme ori gene - Answer allows plasmid replication GFP gene - Answer green fluorescent protein, comes from (source) aequorea vict...

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2 pages

Written in 2024/2025

Grade A+

TestSolver9

what was function of incubation on ice - Answer Incubation slows down the movement of the bilayer in cell. slows fluid cell membrane- fluid mosatic model what was the function of adding calcluim chloride - Answer Addition of calcium chloride solution- the calcium has a positive charger so it shields the negative phosphate in the nucliotide. You nutrelize it so it can go through the cell membrane because it is non polar. Adding the calcium chloride serves to shield the negative charge ...

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8 pages

Written in 2024/2025

Grade A+

TestSolver9

Single-celled; contains only one cell that needs to take up the new gene - Answer To genetically transform an entire organism, you must insert the nee gene into every cell in the organism. Which organism is better suited for the total genetic transformation-- multicellular or single-celled? Quickly; quick assessment to see if trait has been passed on - Answer Scientists often want to know if the genetically transformed organism can pass it's new traits onto its offspring and future g...

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4 pages

Written in 2024/2025

Grade A+

TestSolver9

What is the total volume of reagent in mL? - Answer 0.250mL or 250 microliters pGlo plasmids, when taken up by a bacteria, will code for - Answer a green fluorescence protein GFP Genetic transformation occurs when a cell takes up and expresses a new piece or foreign ____________, often a circular plasmid. - Answer DNA What are 3 real-world links for the study/use of genetic GFP? - Answer study of biological processes, observation of cell movement, use of GFP as a visual mark...

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5 pages

Written in 2024/2025

Grade A+

TestSolver9

1. On which of the plates would you expect to find bacteria most like the original non-transformed E. coli colonies you initially observed? Explain your predictions. - Answer On the -pGLO LB plate you would expect to find bacteria most like the original non-transformed E. coli. This is because everything is allowed to grow inside this plate. 2. If there are any genetically transformed bacterial cells, on which plate(s) would they most likely be located? Explain your predictions. - Answer ...

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3 pages

Written in 2024/2025

Grade A+

TestSolver9

what was function of incubation on ice - Incubation slows down the movement of the bilayer in cell. slows fluid cell membrane- fluid mosatic model what was the function of adding calcluim chloride - Addition of calcium chloride solutionthe calcium has a positive charger so it shields the negative phosphate in the nucliotide. You nutrelize it so it can go through the cell membrane because it is non polar. Adding the calcium chloride serves to shield the negative charge of DNA phosphate groups...

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12 pages

Written in 2022/2023

Grade A+

Tulloch