BIOS 390 (BIOS 390)

BIOS 390BIOS 390 LAB 2: Gel Electrophoresis Gel electrophoresis is a laboratory technique used to separate DNA fragments and other macromolecules by size and charge. Gel electrophoresis is a technique in which fragments of DNA are pulled through a gel matrix by an electric current, and it separates DNA fragments according to size. A standard, or DNA ladder, is typically included so that the size of the fragments in the PCR sample can be determined. The results of a PCR reaction are usually ...

BIOS390 – Molecular Diagnostics Week 1 (TCO 1) The basic principles of genetics contributed by Gregor Johann Mendel are: 1. The principle of segregation 2. The principle of independent assortment (TCO 1) The Augustinian monk, Gregor Johann Mendel, crossed yellow-seeded and green-seeded pea plants and then allowed the offspring to self-pollinate to produce an F2 generation. The results were as follows: 6018 yellow and 2002 green (8020 total). The allele for green seeds has what rel...

Eric Kyle BIOS390 Week 1 & 2 Labs 1 (PCR) & 2 (Gel Electrophoresis)

BIOS 390 Week 6 Exam Study Guide/Assignment Question Set 1 (TCO 8) Which statement is not true about the nucleolus? (Select the correct answer.) (TCO 8) Ribosomes carry out the process of protein synthesis in the _____ after assembly in the _____ within a special compartment called the _____. (TCO 8) Which of the following is not a function of ribosome in protein synthesis? Question Set 2 (TCO 8) What is an anticodon? (TCO 8) Aminoacyl-tRNA synthetases are enzymes that (TCO 8)...

BIOS 390 ERIC KYLE Week 6 Exam Study Guide

BIOS 390 Eric Kyle BIOS390 Week 1 & 2 Labs 1 (PCR) & 2 (Gel Electrophoresis

Once a plasmid has … introduced into competent bacterial cells and the cells have grown into colonies on a medium selective for cells containing the plasmid, the next step is to perform a miniprep of the plasmid DNA in preparation for DNA sequencing. The three steps are: 1) growing cells in liquid culture; 2) purifying the plasmid DNA from the culture; and 3) performing a restriction digest on the purified DNA to determine whether the DNA insert in the plasmid is the … size. To start the liq...

Transcription is the first step in gene expression. It involves copying a gene’s DNA sequence to make an RNA molecule. Before transcription can take place, the DNA double helix must unwind near the gene that is getting transcribed. The region of opened-up DNA is called a transcription bubble. Transcription uses one of the two exposed DNA strands as a template; this strand is called the template strand. The RNA product is complementary to the template strand and is almost identical to the other...

DNA cloning is the process of making multiple, identical copies of a certain piece of DNA. A gene or other DNA fragment of interest is first inserted into a circular piece of DNA called a plasmid. The insertion is done using enzymes that are able to “cut and paste” DNA, and then a molecule of recombinant DNA is produced. Next, the recombinant plasmid is introduced into bacteria. Bacteria carrying the plasmid are selected and grown up. As they reproduce, they replicate the plasmid and pass it...