Introduction to Molecular Biology

a. Define marker b. Define mapping c. Explain the concept of “linkage analysis” d. Explain what a RFLP is and how it is generated e. Differentiate between physical maps, cytogenetic maps and linkage f. Explain why genome mapping are beneficial in finding genes g. Discuss how RFLPs can be used as markers of genetic diseases h. Discuss PCR-RFLP i. Explain what DNA typing is and what it is used for j. Define polymorphism k. Compare and contrast the following DNA markers: STRs, SNPs, Minis...

a. Explain what is a transcriptome b. Differentiate between transient and stable transfection/transformation c. Differentiate between constitutive expression, spatial expression and temporal expression d. Explain what a microarray is e. Explain how a microarray can be used to detect differences in gene expression

1. Define Bioinformatics 2. List the ways in which bioinformatics can be used 3. Discuss Blast results 4. List the various databases for nucleotide and protein sequences