Unit 19 Practical Chemical Analysis

Explaining the function of capillary GC and HPLC components Examples of the use of GC and HPLC in real-life applications Using GC and HPLC chromatograms to determine the analytes in a mixture Discussing how a HPLC method can be developed to determine the amount of specific sugar compound in a sugar Analysing ways to optimise the separation of components from the HPLC method Examples of chromatograms that have been run under different conditions

Finding the concentration of phosphate ions in a solution produced by the action of the enzyme phosphatase Finding the concentration of copper ions present in a solution from a sample of brass Describing the key features of the seven different IR spectra Description of the key features of 1H and 13C NMR spectra and matching them to structural formulas Explaining and analysing the calculation of empirical formula from percentage elemental composition along with determining the molecular formu...

Practical analysis of the copper content in a brass sample, the concentration of bicarbonate in bottled water, and the determination of iron content in an iron tablet. Understandings of primary and secondary standard solutions

Task3: How could HPLC be developed. Discuss how a HPLC method could be developed to determine the amount of a specific sugar molecule in a sugar mixture Task 4: How to optimize separation.

HPLC Chromatograms