MCB 253
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MCB 253 4
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Bradford_Assay_Protocol - University of Illinois, Urbana Champaign MCB 253
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Bradford Assay Protocol Background: The Bradford Assay technique is a quick and efficient way to determine the concentration of a protein in a solution. The way it accomplishes this is to use a blue dye known as Coomassie Brilliant Blue G-250 which when used within an acidic medium will change the color of the protein from brown to blue. According to Bio-Rad, the way it accomplishes this in an acidic medium is "the dye is predominantly in the doubly protonated red cationic form”. When ...
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MCB 253•MCB 253