Centro Escolar University

This document contains must-know information about Tissue Sectioning and Microtomy. This document is for board exam review.

The document contains must-know information about Tissue staining. This document is for board exam review.

The document contains must-know information in tissue staining. This document is for board exam review.

The document contains must-know information about tissue embedding, casting, and blocking. This is for board exam review.

The document contains must-know information about tissue decalcification. This is for board exam review.

The document contains the following: - Draw and label the set-up of Manual paraffin wax infiltration. - Differentiate the 3 types of tissue Impregnation in terms of: - Impregnation Techniques Advantages Disadvantages What is the required volume of embedding medium for routine tissue processing? What is Plastic embedding? What is Double embedding?

The document contains Must Know information about Infiltration, Impregnation, and Embedding. This is for Board Exam Review,

The document contains Must-know information about dealcoholization and clearing for board exam review.

The document contains must-know information about tissue fixation, including effects of fixation, fixation methods , chemical fixatives, 2 types of tissue processing (manual and automated), factors involved in fixation, factors that prolong and accelerate fixation, effects of fixatives in general, problems in fixation, and factors needed to be considered in choosing the right fixative

The document contains a sample quiz in Histology about Epithelial Tissue

The document contains the following: - Draw the different types of embedding molds, disposable and non-disposable - Give the importance of the following sub-steps: Molding, Blocking, Casting - Give 2 uses of ice during blocking. - Enumerate the different types of embedding media.

The document contains answers on the ff. questions: - Which step in H & E staining is for deparaffinnization? - Why are several changes of xylol used at the start of the staining? - Why are several changes of xylol used at the end of staining?

The document contains must-know information about tissue dehydration including the definition of dehydration and Dehydrating agents, their purpose, and characteristics.

The document contains summary tables, Resinous mountant, Aqueous mountant, definition of ringing, labeling, as well as additional information about histopathology.

The document contains answers in the ff.: - Draw the set-up for dehydration and clearing. - Give the importance of dehydration and clearing - Why do you start from lower to higher concentration of alcohol during dehydration? - Enumerate 3 applications of clearing in histopathologic processing. - What will be the effect on the clearing agent if the tissue is incompletely dehydrated? - Give the ideal volume of dehydrating agent and clearing agent

The document contains the following questions: - Enumerate the difficulties encountered during mounting. - What is ringing? - What is the common mounting medium used in mounting the stained sections. - What is the appropriate refractive index of the mounting medium. - Enumerate other mounting medium that can be use in stained sections. - What is the Purpose of mounting?

The document contains answers in the ff. questions: - Name the causes of difficulty in ribbon formation. - Explain why at times sections are rolled.

The document contains answers in the ff. questions: - What is the ideal size and thickness of specimen for tissue processing? - What is the purpose of requiring such size and thickness for tissues processing? - In case of lung specimen, what should be the required size and thickness? - Draw the actual set-up from the time the tissue specimen is received up to the time representative sections are obtained for histopathologic processing.

The document contains the ff.: - Draw the actual setup of fixation. - What is the required volume of fixatives for routine processing? - What are the factors that affect duration of fixation? - Enumerate certain precautions during fixation.

The document contains a sample quiz in histology, together with the answers in red ink.