BIOC0007

BIOC0007 DNA REPAIR EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED What is endonuclease? An enzyme that cleaves the phosphodiester bond within a polynucleotide chain, often used in DNA repair to remove damaged sections. How many DNA errors occur daily in every human cell? Between 10,000 to 1 million DNA errors occur daily in every human cell. What is the role of DNA repair systems in cellular health? they correct the frequent errors that occur in DNA, thereby maintaining cel...

BIOC0007 TRANSCRIPTION EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED RNA polymerase II Eukaryotic; 12 core subunits, 10 essential RNA polymerase Prokaryote; 5 core subunits: ω- stabilises RNA polymerase complex, α1/α2- scaffolding role/protein interaction, β- catalytic site/rNTPs bind, β'- DNA binding, forms transcription bubble Initiation in prokaryotes RNA polymerase binds promoter sequence, binds sigma factor Sigma factor σ1: prevents premature dissociation o...

BIOC0007 LECTURE 1 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED Nucleotide phosphate group, sugar, nitrogen base pentose deoxyribose or ribose 2' hydroxyl group What's the difference between the ribose and deoxyribose? purine a nitrogen base with a double ring structure pyrimidine a nitrogenous base that has a single-ring structure adenine, guanine Name purines cytosine, thymine, uracil Name pyrimidines bases purines and pyrimidines are weak ___ insoluble ...

BIOC0007 QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE where is chromosome loop start replication origin of replication what enzyme replicates the DNA in bacterial chromosomes DNA helicase (DnaB) what enzymes replicate DNA in chromosomal of Eukaryotas ORC how long are RNA primes used in DNA replication 5 to 10 nucleotide base pairs how does replication occur in leading strand continuous replication from 3' to 5' direction how does replication on lagging st...

BIOC0007 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED BRE TFIIB recognition element INR Initiator element Sigma 70 Recognises promoter and secures RNA binding Sigma 1 Prevents premature dissociation Sigma 2 Separates dsDNA and stabilises ssDNA 3.2 region Forms loop between sigma 3 and 4 and helps position incoming dNTPs Preinitiation complex in vitro TBP+TATA; D-TBP binds INR and DPE; A; B binds BRE; F; E; H Termination in prokaryotes: Intrinsic RNA hairpin- loop...

BIOC0007 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE Where does the glycosidic bond form between a nucleobase and the sugar? C1 of sugar and N1 of pyrimidine or N9 of purine Polynucleotide A polymer consisting of many nucleotide monomers in a chain (DNA or RNA) B DNA No. Bases per turn = 10.5 Helix diameter = 20 A Major groove = 13 A Minor groove = 9 A Solid Core Right handed A DNA Dehydrated conditions No. Bases per turn = 11 Helix diameter = 26 A ...

BIOC0007 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE Nucleotide vs nucleoside - Nucleoside- glycosidic linkage of sugar and base - Nucleotide- phosphoester linkage of phosphate to nucleoside Purines Adenine and Guanine Pyrimidines Cytosine and Thymine Nitrogenous bases hydrogen bonding - A-T: 2 H bonds - C-G: 3 H bonds Double helix conformation - Two strands associated via H bonds and Van der Waals - Antiparallel and complementary - 2 strands make fu...