Bio Chem
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Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chromatography - Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid used to remove st...
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Bio chem•Bio chem
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Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chromatography - Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid used to remove st...
This document summarizes key ACS Biochemistry topics, including analytical techniques, purification methods, chemical reagents, and foundational equations such as the Henderson–Hasselbalch equation. It provides clear explanations of chromatography types, electrophoresis methods, and chemical modifications relevant to protein analysis. The content reflects updated material for the 2024/2025 exam cycle and includes accurate question-and-answer style notes.
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Bio chem•Bio chem
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This document summarizes key ACS Biochemistry topics, including analytical techniques, purification methods, chemical reagents, and foundational equations such as the Henderson–Hasselbalch equation. It provides clear explanations of chromatography types, electrophoresis methods, and chemical modifications relevant to protein analysis. The content reflects updated material for the 2024/2025 exam cycle and includes accurate question-and-answer style notes.
HBC 200: BIOCHEMISTRY II END OF YEAR EXAMINATION||A+ GUIDE
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bio-chem•bio-chem
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HBC 200: BIOCHEMISTRY II END OF YEAR EXAMINATION||A+ GUIDE