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The RNase Protection Assay
- Vak
- Instelling
- Boek
The RNase protection assay is a sensitive method for transcription start-site localization. It begins withan RNA probe that is uniformly labeled by incorporation of one [α-32P]NTP, usually [α-32P]UTP. The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates t...
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