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Theorie voor de praktijk

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Leg in het kort de gramkleuring uit, waarop berust het verschil en wat zijn de verschillende stappen van de kleuring?
Leg in het kort de gramkleuring uit, waarop berust het verschil en wat zijn de verschillende stappen...
Bij de gramkleuring wordt er onderscheid gemaakt tussen grampositieve en gramnegatieve bacterien. Het verschil berust op het verschil in celwand bouw. Gram-positieve bacteriën hebben een dikke peptidoglycaanlaag in de celwand die ondoordringbaar is voor ethanol waardoor de kleur niet wordt weggespoeld. De gramnegatieve bacteriën hebben een dunne celwand die de kleurstof niet vasthoudt. Stappen: 1. 1 minuut in kristalviolet. 2. Spoelen met water 3. 1 minuut in lugol oplossing 4. Spoelen met 96% ethanol 5. 1 minuut ontkleuren met 96% ethanol 6. Spoelen met water 7. 1 minuut nakleuren met safranine 8. Afspoelen met water en laten drogen
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