Methylation Study guides, Class notes & Summaries

ASCP MB: Extra-Practice Questions and Answers Scored A The Klenow fragment of E. coli DNA polymerase I is used: - Answer ️️ -to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - Answer ️️ -gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to...

USMLE Biochemistry Exam Questions with Correct Answers Heterochromatin - ANSWER Highly Condensed DNA Transcriptionally inactive Ex. Barr bodies (inactivated X chromosomes) Euchromatin - ANSWER Less condensed DNA Transcriptionally active DNA Methylation - ANSWER Occurs at *CpG Islands* to repress transcription 'CpG Methylation Makes DNA MUTE' Template strand *cytosine* and *adenine* are methylated, which allow mismatch repair enzymes to distingu...

Pyrimidine dimers formed in DNA due to UV radiation can be repaired by? SOS repair Double strand break repair Mismatch repair Photoreactivation repair (nucleotide excision repair) Base excision repair - CORRECT ANSWER Photoreactivation repair (nucleotide excision repair) Which of the following repair mechanisms uses methyl groups to distinguish between parental and daughter strands? Answers: Mismatch repair Photoreactivation repair (Nucleotide excision repair) SOS repair Doub...

The tryptophan operon is a repressible operon that is: - ANSWER-Turned off whenever tryptophan is added to the growth medium. The lactose operon is likely to be transcribed when: - ANSWER-The cyclic AMP and lactose levels are both high within the cell. For a repressible operon to be transcribed, which of the following must occur? - ANSWER-RNA polymerase must bind to the promoter, and the repressor must be inactive. Which of the following, when taken up by the cell, binds to the represso...

ABGC Boards Overview Questions and Answers 100% Solved Angelman Syndrome causes (expression and % of total) ️️**paternal imprinting defect, should be maternal expression 5-7 kb deletion on maternal chr15q11.2-13 (60-70%) UBE3A maternal deletion (11%) Paternal UPD15 (3-7%) Angelman testing strategy and condition features ️️methylation first since finds ~80% of cases then UBE3A seq and del/dup features: happy demeanor, abnormal gait, seizures, delayed/absent speech Prader-Wil...

ETS MAJOR FIELD TEST IN BIOLOGY SENIOR SEMINAR FINAL 2024 QUESTIONS WITH CORRECT ANSWERS ALREADY PASSED AND VERIFIED Pyrimidine dimers formed in DNA due to UV radiation can be repaired by? SOS repair Double strand break repair Mismatch repair Photoreactivation repair (nucleotide excision repair) Base excision repair - CORRECT ANSWER Photoreactivation repair (nucleotide excision repair) Which of the following repair mechanisms uses methyl groups to distinguish between parental and ...

Biochemistry USMLE Step 1 Exam Questions With Answers histones are rich in which 2 AA's -ANSWER- lysine & argenine Barr bodies (what, type of chromatin) -ANSWER- inactive X chromosomes heterochromatin (highly condensed, sterically inaccessible) less condensed, transcriptionally active, sterically accessible chromatin -ANSWER- euchromatin DNA methylation at CpG islands -ANSWER- represses transcription "CpG Methylation makes DNA Mute" DNA methylation (what is methylated and why...

ABGC Boards - Final Review Questions and Answers 100% Solved Hereditary Breast and Ovarian Cancer ️️BRCA 1/2 Breast 50-87% Second primary tumor 50-64% BRCA 1: 20-45% BRCA 2: 10-30% BRCA 2: Male breast 6-10% Li-Fraumeni Syndrome ️️-Sarcoma, breast, leukemia, adrenal and many others -May present in almost every way (ie all family members with same cancers or all with different) -Adrenocortical carcinoma considered pathognomonic -50% have some type by 30, 90% by age 60 -TP53 ...

Pyrimidine dimers formed in DNA due to UV radiation can be repaired by? SOS repair Double strand break repair Mismatch repair Photoreactivation repair (nucleotide excision repair) Base excision repair - CORRECT ANSWER Photoreactivation repair (nucleotide excision repair) Which of the following repair mechanisms uses methyl groups to distinguish between parental and daughter strands? Answers: Mismatch repair Photoreactivation repair (Nucleotide excision repair) SOS repair Doubl...

NR507 Midterm Test Questions with Complete Solutions What is epigenetics? - ANSWER The study of heritable changes in gene expression or phenotype casued by mechanisms *other* than changes in DNA sequences. DNA methylation - ANSWER The attachment of a methyl group to a cytosine base is followed by a guanine base...causes a gene to become transcriptionally inactive or silent. Epigenetic processes play a major role in cancer initiation, progression, and treatmen...