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Exam (elaborations)
SPD Nursing: Decontamination & Sterilization 60-Question Challenge With Correct Answers.
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---5February 20262025/2026A+
- What is the minimum number of air exchanges per hour required in the 
decontamination area? 
10 Correct Answer 
2. In what direction should air flow between the decontamination area and the 
prep and pack area? 
From clean (prep/pack) to dirty (decon) Correct Answer 
3. Which temperature range is mandatory for the decontamination room to inhibit 
microbial growth? 
60°F to 65°F (16°C to 18°C) Correct Answer 
4. What is the first link in the "Chain of Infection" that SPD seeks ...
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trmainanapoleon
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Exam (elaborations)
SPD Nursing Cumulative Mastery Exam(Microbiology, Instrumentation, Case Studies, and AAMI/AORN) With A+ Graded Solutions
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---6February 20262025/2026A+
- What is the specific "Sterility Assurance Level" (SAL) required for a medical 
device to be labeled sterile? Correct Answer 
2. Which bacterial spore is used to challenge the efficacy of a Hydrogen 
Peroxide (VHP) sterilizer? 
Geobacillus stearothermophilus Correct Answer 
3. What is the term for the movement of a liquid through a porous wrap due to 
moisture, which allows bacteria to enter? 
Wicking Correct Answer 
4. In a steam sterilizer, what is the primary consequence of "S...
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trmainanapoleon
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Exam (elaborations)
SPD Nursing Exam: Sterilization Microbiology Test Questions And Revised Solutions
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---6February 20262025/2026A+
- Which microorganism is considered the most resistant to all forms of physical 
and chemical sterilization? 
Prions Correct Answer 
2. What is the name of the protective, "slimy" matrix that bacteria create to shield 
themselves from detergents and disinfectants? 
Biofilm Correct Answer 
3. Which bacterial spore is used as the biological indicator for steam and 
hydrogen peroxide gas plasma sterilization? 
Geobacillus stearothermophilus Correct Answer 
4. What is the biological ind...
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trmainanapoleon
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Exam (elaborations)
SPD Nursing Exam: Instrumentation & Case Studies Questions With A+ Graded Solutions.
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---7February 20262025/2026A+
- Which instrument is characterized by a "tungsten carbide" insert in the jaws, 
usually indicated by gold handles? 
Needle Holder Correct Answer 
2. What is the specific purpose of a "Retractor" during a surgical procedure? 
To hold back edges of an incision or organs to provide exposure to the 
operative site. Correct Answer 
3. How do you distinguish between a "Hemostat" and a "Kelly Clamp"? 
A Hemostat (Mosquito) has serrations along the entire length of the jaw,...
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