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Exam (elaborations)

biology paper 1- 2024
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7

May 2025

2024/2025

A+
Describe and explain how you would use cell fractionation and ultracentrifugation to obtain a sample of nuclei from muscle tissue. [6 marks] - correct answer1. Homogenise (tissue) to break open cells 2. Filter to remove cells debris; 3. Cold (solution) to prevent enzyme activity; 4. keep solution isotonic to prevent organelles bursting 5. Buffered (solution) to stop enzymes/protein denaturing; 6. Centrifuge/spin at low(er) speed so nuclei in pellet Describe how we breathe in. [3 marks]...
innstan
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