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BIOL LAB Final Test Questions and Answers Graded A+
- Exam (elaborations) • 14 pages • 2025
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Describe the basic process of an ELISA. What 
binds to what? When you get a positive or 
negative result from an ELISA, what are you 
actually seeing? - - + Antigen → primary 
antibody → secondary antibody → conjugated 
enzyme --> substrate 
It also involves an antibody or antigen 
(immunologic molecules) that may form an 
antigen-antibody reaction to provide a positive 
result or, if they do not react, a negative result. 
Positive control show color blue = substrate 
binds to conjugate...
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