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BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED
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111
April 2025
2024/2025
A+
Stages of transcription initiation - ANSWER -- holoenzyme recognizes
regulatory sequence in promoter and binds forming the closed promoter
complex - unwind dsDNA to form open promoter complex - transcription initiates (RNA Pol generates short abortive transcripts
that are unstable and released) - at 10nt in length, the complex is stable and turns from initiation to
elongation
Cycling of sigma during transcription - ANSWER -- as Pol transitions
to elongation, sigma factor is relea...
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