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BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED
BCH5413 2
BCH5413 2
Stages of transcription initiation - ANSWER -- holoenzyme recognizes regulatory sequence in promoter and binds forming the closed promoter complex - unwind dsDNA to form open promoter complex - transcription initiates (RNA Pol generates short abortive transcripts that are unstable and released) - at 10nt in length, the complex is stable and turns from initiation to elongation Cycling of sigma during transcription - ANSWER -- as Pol transitions to elongation, sigma factor is relea...
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