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BCH5413 Exam 3 ACTUAL Exam Questions and CORRECT ANSWERs
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---30April 20252024/2025A+
- How does mRNA secondary structure effect translation from a 
polycistronic message? - ANSWER -by blocking translation. 
Ribosomes must bind to linear mRNA in order to proceed with 
translation, however, if an ORF is in the shape of say, a double-stranded 
helix, the ribosome will be unable to bind at this location. Therefore, 
there may be more proteins synthesized from one ORF that is linear and 
unblocked compared to another ORF on the same mRNA that is not in a 
linear shape. 
 
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