CBIO 233 (Microbiology)

These labs covered basic microbiology techniques, including smear preparation, simple staining, and bright-field microscopy to observe bacteria. Media preparation and sterilization were performed to support microbial growth. Gram staining was used to differentiate bacteria by cell wall structure, also spread & streak plate methods were used.

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4 items

latiffaliburd

This lab focused on using the spread plate and streak plate techniques to isolate individual bacterial colonies. These methods help dilute a microbial sample across an agar surface to achieve separation and allow for pure culture growth.

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3 pages

Written in 2016/2017

latiffaliburd

This lab involved performing a Gram stain to differentiate bacteria based on cell wall structure. Using a series of dyes and a decolorizer, bacteria were classified as Gram-positive or Gram-negative and observed under a microscope.

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4 pages

Written in 2016/2017

latiffaliburd

This lab focused on preparing culture media and sterilizing it using autoclaving to create a sterile environment for microbial growth. Proper media preparation and sterilization techniques are critical for preventing contamination in microbiological environments.

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2 pages

Written in 2016/2017

latiffaliburd

This experiment focused on the preparation of bacterial smears and the application of simple staining to observe microbial cell morphology under a bright-field microscope. Aseptic techniques were used to prepare smears, which were then stained using a basic dye to enhance contrast. The techniques practiced are essential for basic microbial identification.

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4 pages

Written in 2016/2017

latiffaliburd