CBIO 233 (Microbiology)

This lab focused on using the spread plate and streak plate techniques to isolate individual bacterial colonies. These methods help dilute a microbial sample across an agar surface to achieve separation and allow for pure culture growth.

This lab involved performing a Gram stain to differentiate bacteria based on cell wall structure. Using a series of dyes and a decolorizer, bacteria were classified as Gram-positive or Gram-negative and observed under a microscope.

This lab focused on preparing culture media and sterilizing it using autoclaving to create a sterile environment for microbial growth. Proper media preparation and sterilization techniques are critical for preventing contamination in microbiological environments.

This experiment focused on the preparation of bacterial smears and the application of simple staining to observe microbial cell morphology under a bright-field microscope. Aseptic techniques were used to prepare smears, which were then stained using a basic dye to enhance contrast. The techniques practiced are essential for basic microbial identification.