ACS BIOCHEMISTRY - OBU
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Separation of Organelles / Fractionation of Cells - ANSWER Differential Centrifugation 1. Tissue Homogenization 2. Low speed centrifugation (whole cells, nuclei, membrane) 3. Medium speed centrifugation (mitochondria, lysosomes, peroxisomes) 4. High speed centrifugation (microsomes -fragments of ER) 5. Very high-speed centrifugation (soluble proteins) Isopycnic Centrifugation - ANSWER Sucrose-Density (components separated by density) Interactions occur... - ANSWER because of the elec...
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Separation of Organelles / Fractionation of Cells - ANSWER Differential Centrifugation 1. Tissue Homogenization 2. Low speed centrifugation (whole cells, nuclei, membrane) 3. Medium speed centrifugation (mitochondria, lysosomes, peroxisomes) 4. High speed centrifugation (microsomes -fragments of ER) 5. Very high-speed centrifugation (soluble proteins) Isopycnic Centrifugation - ANSWER Sucrose-Density (components separated by density) Interactions occur... - ANSWER because of the elec...