Why is the mouth of the test tube passed over the flame after opening and before
closing the tube cap?
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to kill bacteria surrounding the test tube top and mouth
To prevent bacteria entering from the air
In which hand should the tube cap be held while transferring cultures from one
medium to another?
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dominant hand
,Are acid-fast negative cells stained by carbolfuchsin? If so, how can this be a
differential stain?
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Yes, carbolfuchsin stains all cells. Non acid-fast cells lose their color when
you apply acid/alcohol reagent; need to apply methylene blue
counterstain.
How do you calculate total magnification
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multiply occular lens x objective lens
Why does this exercise call for an older (5-day) culture of Bacillus?
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bacteria begins to form endospores when all nutrients are depleted in the
medium before they completely starve.
Stains used in endospore staining
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Malachite green - endospores (appear green)
Safranin - vegetative cells (appear pink/red)
Ingredients in Nutrient Broth
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beef extract (3.0 g)
peptone (5.0 g)
distilled or deionized water (1.0 L)
Alternative names for reagents
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Crystal violet - primary stain
Iodine - mordant
Alcohol/acetone solution - decolorizer
safranin - counter stain
Endospore staining procedure
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closing the tube cap?
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to kill bacteria surrounding the test tube top and mouth
To prevent bacteria entering from the air
In which hand should the tube cap be held while transferring cultures from one
medium to another?
Give this one a try later!
dominant hand
,Are acid-fast negative cells stained by carbolfuchsin? If so, how can this be a
differential stain?
Give this one a try later!
Yes, carbolfuchsin stains all cells. Non acid-fast cells lose their color when
you apply acid/alcohol reagent; need to apply methylene blue
counterstain.
How do you calculate total magnification
Give this one a try later!
multiply occular lens x objective lens
Why does this exercise call for an older (5-day) culture of Bacillus?
Give this one a try later!
bacteria begins to form endospores when all nutrients are depleted in the
medium before they completely starve.
Stains used in endospore staining
, Give this one a try later!
Malachite green - endospores (appear green)
Safranin - vegetative cells (appear pink/red)
Ingredients in Nutrient Broth
Give this one a try later!
beef extract (3.0 g)
peptone (5.0 g)
distilled or deionized water (1.0 L)
Alternative names for reagents
Give this one a try later!
Crystal violet - primary stain
Iodine - mordant
Alcohol/acetone solution - decolorizer
safranin - counter stain
Endospore staining procedure
Give this one a try later!
