MGY 250 Unit 8 Quiz Questions and Answers Already Passed
Which of the following statements are true of the CRISPR system: (select all that apply)
The CRISPR locus is easy to modify using RNA engineering and can be designed to cut any
desired double stranded DNA sequence
A bacterial CRISPR locus consists of repetitive sequences interspaced with viral DNA sequence
The Cas9 enzyme recognizes DNA sequences using a guide RNA
Both the CRISPR locus and 'cas' genes are part of a bacterial immune system - Answers A
bacterial CRISPR locus consists of repetitive sequences interspaced with viral DNA sequence
The Cas9 enzyme recognizes DNA sequences using a guide RNA
Both the CRISPR locus and 'cas' genes are part of a bacterial immune system
According to Dr. van der Kooy, current therapeutic treatment with stem cells include (select all
that apply):
Using skin stem cells to grow sheets of skin that can replace burnt skin
Using Yamanaka factors to reprogram leukaemia cells to pluripotent, non-cancerous cells
Using heart stem cells to regenerate heart tissue damaged by cardiac arrest
Using blood stem cells to replenish blood cells in patients with leukaemia who have had their
native supply blood cells eradicated by chemotherapy - Answers Using skin stem cells to grow
sheets of skin that can replace burnt skin
Using blood stem cells to replenish blood cells in patients with leukaemia who have had their
native supply blood cells eradicated by chemotherapy
Dr. van der Kooy described an experiment performed by Dr. Janet Rossant and colleagues in
which mouse embryonic stem cells expressing green fluorescent protein were injected into a
pseudo-pregnant mouse. Using a "genetic trick" the host cells were segregated to the pre-
placental tissue and only the injected cells were in the inner cell mass, such that the mouse
eventually born was completely green. This experiment demonstrated that:
Embryonic stem cells can generate every cell in the body in vivo
, Embryonic stem cells can generate chimeric organisms
Embryonic stem cells can contribute to the placenta
Embryonic stem cells do not interfere with the growth of a mouse - Answers Embryonic stem
cells can generate every cell in the body in vivo
iPS cells can be used to (select all that apply):
Model genetic disease by exploring the effect of a particular mutation on the development of
different tissue types
Test drug toxicity
Model genetic disease by exploring the effect of a particular mutation on the development of a
human
Differentiate into different cell types - Answers Model genetic disease by exploring the effect of
a particular mutation on the development of different tissue types
Test drug toxicity
Differentiate into different cell types
Dr. van der Kooy describes how gene therapy is in clinical trials for the delivery of normal copies
of RPE65 to retinal pigment epithelial (RPE) cells that have a pathogenic mutation. One
limitation of this strategy is:
It is very expensive and unavailable in Canada
The pathogenic mutation in RPE65 causes other important vision cells to die
Preliminary studies in dogs demonstrated that vision lost still occurred
The pathogenic mutation in RPE65 is typically only discovered when vision is impaired and RPE
cells have already died. Thus, the best this strategy can do is reduce further vision loss -
Answers The pathogenic mutation in RPE65 is typically only discovered when vision is impaired
and RPE cells have already died. Thus, the best this strategy can do is reduce further vision loss
In Dr. Cohn's experience, even in the absence of available treatment for a rare genetic disease
having a diagnosis is important because:
Which of the following statements are true of the CRISPR system: (select all that apply)
The CRISPR locus is easy to modify using RNA engineering and can be designed to cut any
desired double stranded DNA sequence
A bacterial CRISPR locus consists of repetitive sequences interspaced with viral DNA sequence
The Cas9 enzyme recognizes DNA sequences using a guide RNA
Both the CRISPR locus and 'cas' genes are part of a bacterial immune system - Answers A
bacterial CRISPR locus consists of repetitive sequences interspaced with viral DNA sequence
The Cas9 enzyme recognizes DNA sequences using a guide RNA
Both the CRISPR locus and 'cas' genes are part of a bacterial immune system
According to Dr. van der Kooy, current therapeutic treatment with stem cells include (select all
that apply):
Using skin stem cells to grow sheets of skin that can replace burnt skin
Using Yamanaka factors to reprogram leukaemia cells to pluripotent, non-cancerous cells
Using heart stem cells to regenerate heart tissue damaged by cardiac arrest
Using blood stem cells to replenish blood cells in patients with leukaemia who have had their
native supply blood cells eradicated by chemotherapy - Answers Using skin stem cells to grow
sheets of skin that can replace burnt skin
Using blood stem cells to replenish blood cells in patients with leukaemia who have had their
native supply blood cells eradicated by chemotherapy
Dr. van der Kooy described an experiment performed by Dr. Janet Rossant and colleagues in
which mouse embryonic stem cells expressing green fluorescent protein were injected into a
pseudo-pregnant mouse. Using a "genetic trick" the host cells were segregated to the pre-
placental tissue and only the injected cells were in the inner cell mass, such that the mouse
eventually born was completely green. This experiment demonstrated that:
Embryonic stem cells can generate every cell in the body in vivo
, Embryonic stem cells can generate chimeric organisms
Embryonic stem cells can contribute to the placenta
Embryonic stem cells do not interfere with the growth of a mouse - Answers Embryonic stem
cells can generate every cell in the body in vivo
iPS cells can be used to (select all that apply):
Model genetic disease by exploring the effect of a particular mutation on the development of
different tissue types
Test drug toxicity
Model genetic disease by exploring the effect of a particular mutation on the development of a
human
Differentiate into different cell types - Answers Model genetic disease by exploring the effect of
a particular mutation on the development of different tissue types
Test drug toxicity
Differentiate into different cell types
Dr. van der Kooy describes how gene therapy is in clinical trials for the delivery of normal copies
of RPE65 to retinal pigment epithelial (RPE) cells that have a pathogenic mutation. One
limitation of this strategy is:
It is very expensive and unavailable in Canada
The pathogenic mutation in RPE65 causes other important vision cells to die
Preliminary studies in dogs demonstrated that vision lost still occurred
The pathogenic mutation in RPE65 is typically only discovered when vision is impaired and RPE
cells have already died. Thus, the best this strategy can do is reduce further vision loss -
Answers The pathogenic mutation in RPE65 is typically only discovered when vision is impaired
and RPE cells have already died. Thus, the best this strategy can do is reduce further vision loss
In Dr. Cohn's experience, even in the absence of available treatment for a rare genetic disease
having a diagnosis is important because:
