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Exam (elaborations)

PCB 3063 UF EXAM 2 QUESTIONS AND VERIFIED ANSWERS

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PCB 3063 UF EXAM 2 QUESTIONS AND VERIFIED ANSWERS

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PCB 3063
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PCB 3063











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Institution
PCB 3063
Course
PCB 3063

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Uploaded on
December 15, 2025
Number of pages
58
Written in
2025/2026
Type
Exam (elaborations)
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Questions & answers

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polysomes


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are mRNAs with several ribosomes translating at once




rDNA


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rRNA genes are moderately repetitive and randomly repeated




Polyploidy

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occurs when more than two sets of chromosomes are present




GAL genes of yeast


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-inducible by the presence of galactose but only if the concentration of
glucose is low
-subject to catabolite repression
-the GAL 1 and GAL 10 genes are controlled by a central control region,
UASG, that contains four binding sites for the Gal 4 protein




mRNA


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template for protein synthesis




HDAC


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deacetylates histones, decreased transcription

,Activity of SXL


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-the sex lethal (Sxl), transformer (tra), and doublesex (dsx) genes are part of
a hierarchy of sex determination in Drosophila
-the Sxl gene acts as a switch that selects the pathway of sexual
development by controlling splicing of dsx transcript in a female-specific
fashion




Luria Delbruck test


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demonstrated that mutations are not adaptive but occur spontaneously




Initiation of translation in prokaryotes


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IF1 and IF3 interact with 30s ribosomal subunit
mRNA binds
fMet tRNA binds
50s subunit sandwiches mRNA in ribosome and IFs released




RNA polymerase


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, directs the synthesis of RNA using a DNA template. No primer is required.
the enzyme uses ribonucleotides instead of deoxyribonucleotides.




SOS repair system


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-allows DNA synthesis to become error-prone
-is mutagenic but may allow the cell to survive DNA damage that may
otherwise kill it




Postreplication repair


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occurs when DNA replication skips over a lesion and requires homologous
recombination mediated by the RecA protein
-MutS recognizes the mismatched pair
-MutH recognizes methylated parent strand and nicks daughter strand
-new strand is excised and replaced between nick and mismatched pair




RNA pol 2


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