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PCB3134 Final Exam – Questions With Clear Solutions

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PCB3134 Final Exam – Questions With Clear Solutions

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Terms in this set (131)


What type of microscopy uses Light (Brightfield) Microscopy
visible light?

What is the resolution limit of ~200-350 nm
light microscopy?

Which microscopy technique Transmission Electron Microscopy (TEM)
reveals internal ultrastructure?

What does Scanning Electron 3D surface topology
Microscopy (SEM) produce?

What is the focus of cytology? Cell structure

What method uses Subcellular fractionation
centrifugation to isolate
organelles?

What are the key techniques DNA sequencing, Recombinant DNA, Transformation, PCR
used in genetics?

What are the classes of Enzymes, Structural, Motility, Regulatory, Transport, Signaling,
proteins? Receptor, Defensive, Storage

What is the generic structure of Central α-carbon, Amino group (NH₂), Carboxyl group (COOH),
an amino acid? R-group (side chain), Hydrogen

Which amino acids are classified Ala, Val, Leu, Ile, Met, Phe, Trp, Pro, Gly
as nonpolar?

What is the directionality of From N-terminus (free amino group) to C-terminus (free carboxyl
protein synthesis? group)

What type of bonds stabilize Disulfide bonds, Hydrogen bonds, Ionic interactions, Van der
protein structure? Waals forces, Hydrophobic interactions

What is the primary structure of Amino acid sequence
a protein?

What are the two types of α-helices and β-sheets
secondary structures in
proteins?

What are the characteristics of Genetic storage, Antiparallel strands, Right-handed helix,
DNA? Complementary base pairing

What are the two types of Purines (Adenine, Guanine) and Pyrimidines (Cytosine, Thymine,
nucleic acid bases? Uracil)

What is the role of glycosidic Link monosaccharides
bonds?

, What are the two types of Glycogen (animals) and Starch (plants)
glucose polymers?

What are the major classes of Fatty acids, Triacylglycerols, Phospholipids, Glycolipids, Steroids,
lipids? Terpenes

What is the primary function of Membrane structure
phospholipids?

What are the functions of Energy storage
triacylglycerols?

What distinguishes α and β They differ at the anomeric carbon.
anomers in sugars?

What type of linkages does β(1→4) linkages
cellulose have?

What is the significance of Lipids spontaneously form liposomes in water.
liposomes?

What model describes the Fluid mosaic model
structure of cell membranes?

What are the major components Phospholipids, glycolipids, cholesterol, proteins
of cell membranes?

What is the role of cholesterol in At high temperatures, it decreases fluidity; at low temperatures, it
membrane fluidity? increases fluidity.

What are integral membrane Proteins that span the membrane or are embedded deeply within
proteins? it.

What is the function of They bind to the membrane surface via electrostatic interactions.
peripheral membrane proteins?

What is the significance of It provides protection, aids in cell recognition, stabilizes proteins,
glycosylation in membrane and facilitates cell-cell communication.
proteins?

What is the difference between Simple diffusion does not require proteins, while facilitated
simple diffusion and facilitated diffusion requires transport proteins.
diffusion?

What is the primary function of To accelerate reactions by lowering activation energy.
enzymes?

What is the transition state in A high-energy intermediate that enzymes help stabilize.
enzymatic reactions?

What does the Michaelis- The relationship between reaction rate and substrate
Menten equation describe? concentration in enzyme kinetics.

What does a low Km value It indicates high affinity.
indicate about an enzyme's
affinity for its substrate?

What are prosthetic groups in Nonprotein components required for full enzyme activity, such
enzymes? as metal ions or coenzymes.

What happens to enzymes at They may denature and lose their functionality.
high temperatures?

What is the role of the active site It is the pocket where the substrate binds and where the reaction
in an enzyme? occurs.

What is the induced-fit model of It describes how an enzyme changes shape upon substrate
enzyme action? binding to stabilize the transition state.

What is the difference in DNA Bacteria have a single circular chromosome; Archaea have
organization between Bacteria, circular DNA with histones; Eukaryotes have multiple linear
Archaea, and Eukaryotes? chromosomes with histones.

How do Bacteria and Archaea They divide by binary fission.
divide?

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