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MICROBIOLOGY LAB FINAL REVIEW
QUESTIONS WITH DETAILED VERIFIED
ANSWERS
What type of media would you use to allow growth of many bacterials
species from the sample enviornment? Ans: TSA
triptic soy agar
Where would you incubate a soil sample?
Mouth/touch plate?
Sample from frozen tundra why? Ans: soil - 22-25
mouth/touch- 37
Frozen tundra- 4
For proper growth to occur, the sample must be incubated/refrigerated at
the temperature its usual environment is.
Aseptic Technique: transferring cultures from liquid to plate or liquid to
liquid Ans: -Hold inoculating loop in flame for 2-3 seconds while red hot
-remove the cap from the broth culture and flame the mouth of the tube
by passing it through the flame
-Insert loop and withdraw
-re-flame mouth of tube, and cap it
-touch the loop to the plate and streak it back and forth a few times
-cover plate & reflame loop
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Define Resolution Ans: The ability to distinguish two small objects apart
from each other.
Define Parfocal Ans: When you focus on a specimen under a low
magnification, you can switch the objective to a higher magnification and
your specimen will be almost in focus.
Define Magnification Ans: -enlarging an image.
can be calculated by multiplying the magnification of the objective lens
by the magnification of the eye piece
Eyepiece Ans: the lens or group of lens that is closest to the eye in a
microscope
Objective Lens Ans: The lens or system of lens in a microscope that is
nearest to the object being viewed
Stage Ans: a small platform on a microscope where the specimen is
mounted for examination
Iris Diaphragm Ans: regulates amount of light admitted to a lens
Light Source Ans: provides light to microscope
Stage control Ans: controls the stage position (up or down)
On/Off button Ans: turns microscope on and off
Coarse focus Ans: focuses the speciman on stage
Fine Focus Ans: fine tunes the focus of the specimen and various parts
of the specimen
What is the purpose of heat fixing after the smear has air dried? Ans: to
make the bacteria adhere to the slide
Purpose of a simple stain Ans: stains make otherwise transparent
microorganisms visible under a microscope.
MICROBIOLOGY LAB FINAL REVIEW
QUESTIONS WITH DETAILED VERIFIED
ANSWERS
What type of media would you use to allow growth of many bacterials
species from the sample enviornment? Ans: TSA
triptic soy agar
Where would you incubate a soil sample?
Mouth/touch plate?
Sample from frozen tundra why? Ans: soil - 22-25
mouth/touch- 37
Frozen tundra- 4
For proper growth to occur, the sample must be incubated/refrigerated at
the temperature its usual environment is.
Aseptic Technique: transferring cultures from liquid to plate or liquid to
liquid Ans: -Hold inoculating loop in flame for 2-3 seconds while red hot
-remove the cap from the broth culture and flame the mouth of the tube
by passing it through the flame
-Insert loop and withdraw
-re-flame mouth of tube, and cap it
-touch the loop to the plate and streak it back and forth a few times
-cover plate & reflame loop
, Page | 2
Define Resolution Ans: The ability to distinguish two small objects apart
from each other.
Define Parfocal Ans: When you focus on a specimen under a low
magnification, you can switch the objective to a higher magnification and
your specimen will be almost in focus.
Define Magnification Ans: -enlarging an image.
can be calculated by multiplying the magnification of the objective lens
by the magnification of the eye piece
Eyepiece Ans: the lens or group of lens that is closest to the eye in a
microscope
Objective Lens Ans: The lens or system of lens in a microscope that is
nearest to the object being viewed
Stage Ans: a small platform on a microscope where the specimen is
mounted for examination
Iris Diaphragm Ans: regulates amount of light admitted to a lens
Light Source Ans: provides light to microscope
Stage control Ans: controls the stage position (up or down)
On/Off button Ans: turns microscope on and off
Coarse focus Ans: focuses the speciman on stage
Fine Focus Ans: fine tunes the focus of the specimen and various parts
of the specimen
What is the purpose of heat fixing after the smear has air dried? Ans: to
make the bacteria adhere to the slide
Purpose of a simple stain Ans: stains make otherwise transparent
microorganisms visible under a microscope.
