Acs biochemistry first semester exam questions and answers / graded a

ACS Biochemistry First Semester




ACS Biochemistry First

Semester






, ACS Biochemistry First Semester




Buffer Order and Henderson Hasselbach: Water, Buffer, Salt, Base pH =

pKa + log(A/HA)

pKa decrease as temp increase so pH decrease

1. Ramachandran plots: ¦ = N-R È = C-R

optimal angle to prevent steric hinderance

2. Protein structure of a helix, B sheet, reverse turn: a: LEMARKHQ

B: VICYFTW

RT: PSDNG

3. how many amino acids in alpha turn helix: 3.6

4. Super secondary structure: helix turn helix

5. parallel vs. antiparallel: parallel wedges face same way antiparallel face

opposite






, ACS Biochemistry First Semester




6. Isoelectric point (pI): pH at which a particular

molecule carries no net electrical charge.




7. Protein Separation Techniques (ALL): Homogenization

Salting Out

Affinity Chromatgraphy

Ion Exchange

Size Exclusion/Gel Filtration

H, S, A, I, SG

He said all individuals SinG

8. Protein Separation Techniques (H): Homogenization disrupt cells, sonicator,

centrifuge

9. Protein Separation Techniques (S): Salting out