BIOL 107 Lab Final Exam Questions and Answers
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Terms in this set (45)
What is a negative A group in which the independent variable is absent
control?
When the p-value obtained is less than 0.05 (This
When should you reject
means that the results of the experiment are
your null hypothesis?
statistically significant.)
What was the control in The control in this experiment was water in place of
Lab 4 (the experiment that smoke treatment and it was a negative control.
studied the effects of
smoke treatment on
Tetrahymena cells)? What
kind of control was it?
Why was ONPG used to ONPG is a substrate that is similar to lactose and
test enzyme activity? easier to measure.
Enzymes are made up of proteins, which fold and
Why did lower (more bond to create a 3D structure, forming an active site
acidic) pHs not bind to where enzymes can bind. Different pHs affect protein
ONPG and result in lower folding and these lower pHs caused the proteins not
lactase activity? to fold correctly and therefore the ONPG could not
bind properly to the active site.
, Sugar cane contains large amounts of glucose, which
makes it effective for the production of CO2 through
Why was sugar cane used
fermentation. Since it is known that sugar cane will
as a control in Lab 6 (the
result in high rates of fermentation, it makes for a
biofuels experiment)?
good control in this experiment to compare to other
groups that are not yet known to be effective or not.
Why are second- Many ethical and environmental concerns surround
generation biofuels being the use of food crops as sources of biofuels, such as
researched as alternatives the driving up of food prices due to increased
to plant materials like demand and deforestation due to sugar cane crops
sugar cane and corn? being planted in cleared parts of the rainforest.
Cellulase breaks down cellulose, which in the
experiment in Lab 6, the yeast was unable to do on its
Why does cellulase
own because it does not have enzymes that can break
treatment increase the
down cellulose. Microfibrils are what make up
rate of fermentation?
cellulose and have very complex structures that make
What other step had to be
it difficult for cellulase to reach the cellulose it down
completed in Lab 6 before
into glucose, so pre-treatment using heat or
cellulase treatment?
mechanical methods must be completed first to break
up the cell wall.
What is the difference An absorption spectrum determines which
between an absorption wavelengths of light are absorbed by chloroplast
spectrum and an action pigments and an action spectrum demonstrates which
spectrum in regards to the colours of light result in the highest rates of
photosynthesis photosynthesis.
experiment in Lab 7?
DCPIP is an indicator dye that changes colour when
Why was DCPIP used in reduced (gain of electrons). It goes from being blue
Lab 7 to measure the rate when in its oxidized form to colourless in its reduced
of photosynthesis? form. The less colour loss in DCPIP, the higher rate of
photosynthesis.
100% Solved
Save
Terms in this set (45)
What is a negative A group in which the independent variable is absent
control?
When the p-value obtained is less than 0.05 (This
When should you reject
means that the results of the experiment are
your null hypothesis?
statistically significant.)
What was the control in The control in this experiment was water in place of
Lab 4 (the experiment that smoke treatment and it was a negative control.
studied the effects of
smoke treatment on
Tetrahymena cells)? What
kind of control was it?
Why was ONPG used to ONPG is a substrate that is similar to lactose and
test enzyme activity? easier to measure.
Enzymes are made up of proteins, which fold and
Why did lower (more bond to create a 3D structure, forming an active site
acidic) pHs not bind to where enzymes can bind. Different pHs affect protein
ONPG and result in lower folding and these lower pHs caused the proteins not
lactase activity? to fold correctly and therefore the ONPG could not
bind properly to the active site.
, Sugar cane contains large amounts of glucose, which
makes it effective for the production of CO2 through
Why was sugar cane used
fermentation. Since it is known that sugar cane will
as a control in Lab 6 (the
result in high rates of fermentation, it makes for a
biofuels experiment)?
good control in this experiment to compare to other
groups that are not yet known to be effective or not.
Why are second- Many ethical and environmental concerns surround
generation biofuels being the use of food crops as sources of biofuels, such as
researched as alternatives the driving up of food prices due to increased
to plant materials like demand and deforestation due to sugar cane crops
sugar cane and corn? being planted in cleared parts of the rainforest.
Cellulase breaks down cellulose, which in the
experiment in Lab 6, the yeast was unable to do on its
Why does cellulase
own because it does not have enzymes that can break
treatment increase the
down cellulose. Microfibrils are what make up
rate of fermentation?
cellulose and have very complex structures that make
What other step had to be
it difficult for cellulase to reach the cellulose it down
completed in Lab 6 before
into glucose, so pre-treatment using heat or
cellulase treatment?
mechanical methods must be completed first to break
up the cell wall.
What is the difference An absorption spectrum determines which
between an absorption wavelengths of light are absorbed by chloroplast
spectrum and an action pigments and an action spectrum demonstrates which
spectrum in regards to the colours of light result in the highest rates of
photosynthesis photosynthesis.
experiment in Lab 7?
DCPIP is an indicator dye that changes colour when
Why was DCPIP used in reduced (gain of electrons). It goes from being blue
Lab 7 to measure the rate when in its oxidized form to colourless in its reduced
of photosynthesis? form. The less colour loss in DCPIP, the higher rate of
photosynthesis.
