Laboratory Animal Science
MKV 2024-2025
1. You perform an experiment with the Hanta virus. One of the researchers develops
flu-like symptoms after working with the animals. What do you do?
o Isolate the researcher and perform a medical investigation
o Stop the experiment
o Increase the frequency of cage cleaning
o Do genetic testing on the animals
2. What is a good definition of heterosis (F1 hybrid strain)?
o Higher viability than parenteral strains
o Random mix of alleles from parenteral strains
3. Monkeys case. Food. Overcrowded?
o Some tests can only be conducted on animals that bear a strong
similarity to humans, such as primates (we use monkeys for research
into the functions of the brain)
4. Hoe zijn de tanden van een muis (rodents) verdeeld?
o (1I, 0C, 0P, 3M) x4 ➔ 16
5. What is the Lee-Boot effect (muis en rat)?
o Females kept in group: tendency to anoestrus
o A male is introduced: oestrus synchronization (Whitten effect)
o After a successful mating, another male is introduced into the cage within 24
hours: no implantation (Bruce effect)
6. What animals are protected under legislation?
○ Vertebrates (living) & cephalopoda
○ Mammals
○ Only vertebrates
o Vertrebrates & invertebrates
7. How many generations of brother x sister-breeding do you need for an inbred strain?
o 20
o 5
o 10
o 15
8. What is not a humane endpoint?
o Death
o Blue extremities
o Loss until 20% weight (since the begin of the experiment)
o No appetite
1
,9. You want to do mild surgery on mice. What is the best anesthetic for easy adjustment
and quick recovery?
o Isoflurane inhalation
o Ketamine/xylazine injection
o Halothane inhalation
o Thiopental injection
10. What is the 4th R in ‘the principles of humane experimental technique’?
o Responsibility
o Replace
o Reduce
o Refine
11. What is the advantage of a ‘closed formula’ regarding the natural feed?
o Better taste
o Lower contamination risk
o No differences between batches
12. What is a diastema?
o Gap between the molars and incisors
13. How to prepare an operation (pre-operative prevention)?
o Inside out disinfect (in circle motion) the animal
o Outside in disinfect the animal
o With triangular shaving
o Drape must be used on the animal and the rest of your equipment
14. What is the optimal water temperature for zebrafish (danio rerio)?
o 27-29°C (28,5°C)
15. What is redirected behavior (conflict behavior)?
o Aggression shifts from the initial object toward other objects
o More playful behaviour
o Aggression to a new object
o Stereotypical behaviour
16. What are the steps of health screening next to pre-necropsy and necropsy?
o Bacteriology, serology and parasitology
o PCR and indirect methods
o PCR, serology and parasitology
17. Why do you perform pilot studies?
o To look at the feasibility and refine the experiment
o To decrease the number of animals used
18. Wat is de meest voorkomende bijwerking van NSAID’s gebruiken bij proefdieren?
o Gastric ulcers
19. Welke medicatie wordt als alfa-adrenerge agonist gebruikt?
o Metaraminol (ook: xylazine en medetomidine)
20. Welke medicatie wordt als lokaal anestheticum gebruikt bij muizen?
o Xylocaine (lidocaine)
21. Wat is de gestatie periode van een oryctolagus cuniculus (konijn)?
o 30-35 dagen (31 dagen)
22. In een experiment gaan zebravissen dood. Wat is de meest waarschijnlijke reden?
o Slechte water kwaliteit
2
, 23. Wat is een manier om allergieën te voorkomen?
o Investeer in ingenieurtechnieken zoals goede ventilatie en filtratie
24. Wat is het grote voordeel van F1-hybrids (inbred strains)?
o Bezitten meer ‘hybrid vigor’ dan hun ouders
o Makkelijker te fokken
25. What are gnotobionts?
o Condition of animals that are germ-free or have a known and controlled
set of microorganisms living in or on them (harbour a fully known
microflora)
26. Waarvoor gebruik je een non-technical summary (NTS)?
o An NTS is a brief summary of an animal research project written in
clear, accessible language for the general public
27. Wat doet een animal welfare body (AWB) niet?
o Conducting animal experiences
o Supervision
o Opvolgen van approval
o Make sure the 3 R's are known by everyone
28. Wat is de impact van chronisch stress op de hippocampus bij muizen?
o Daling neurogenese
o Stijging neurogenese
o Geen
29. Wat is het voordeel van inhalatie anesthesie(t.o.v. injectie anesthesie)?
o Precise control over the depth/duration of anesthesia an fast recovery
o Less equipment
o Cheaper
o All of the above
30. Wat is de reden dat dieren ziek worden?
o All of the above
o Slecht omgeving
o Pathogenen
31. Wat is de moeilijkheid om inbred muizen consistent te houden?
o Genetisch drift
32. Wat is het gevolg als je muizen sociaal onopgelost conflict hebben (environmental
factors that disturb the homeostasis when an animal cannot adapt)?
o Stress (and decreased well-being)
33. Casus: Je hebt varkens waarbij je ischemie gaat induceren, ze krijgen de correcte
anaesthesie. Na de operatie krijgen ze 1 week analgesie. Vervolgens wordt er een
product (medicatie) ingespoten om te kijken of dit zou helpen. Gedurende 3 maanden
worden ook ECG’s afgenomen. Wat is de severity van dit onderzoek?
o Moderate (surgery and post-operative pain)
o Mild
o Post-mortem
o Non-recovery
34. What hormone is released fast when the animal experiences acute stress?
o Cortisol (and ACTH)
o Estrogen
3
MKV 2024-2025
1. You perform an experiment with the Hanta virus. One of the researchers develops
flu-like symptoms after working with the animals. What do you do?
o Isolate the researcher and perform a medical investigation
o Stop the experiment
o Increase the frequency of cage cleaning
o Do genetic testing on the animals
2. What is a good definition of heterosis (F1 hybrid strain)?
o Higher viability than parenteral strains
o Random mix of alleles from parenteral strains
3. Monkeys case. Food. Overcrowded?
o Some tests can only be conducted on animals that bear a strong
similarity to humans, such as primates (we use monkeys for research
into the functions of the brain)
4. Hoe zijn de tanden van een muis (rodents) verdeeld?
o (1I, 0C, 0P, 3M) x4 ➔ 16
5. What is the Lee-Boot effect (muis en rat)?
o Females kept in group: tendency to anoestrus
o A male is introduced: oestrus synchronization (Whitten effect)
o After a successful mating, another male is introduced into the cage within 24
hours: no implantation (Bruce effect)
6. What animals are protected under legislation?
○ Vertebrates (living) & cephalopoda
○ Mammals
○ Only vertebrates
o Vertrebrates & invertebrates
7. How many generations of brother x sister-breeding do you need for an inbred strain?
o 20
o 5
o 10
o 15
8. What is not a humane endpoint?
o Death
o Blue extremities
o Loss until 20% weight (since the begin of the experiment)
o No appetite
1
,9. You want to do mild surgery on mice. What is the best anesthetic for easy adjustment
and quick recovery?
o Isoflurane inhalation
o Ketamine/xylazine injection
o Halothane inhalation
o Thiopental injection
10. What is the 4th R in ‘the principles of humane experimental technique’?
o Responsibility
o Replace
o Reduce
o Refine
11. What is the advantage of a ‘closed formula’ regarding the natural feed?
o Better taste
o Lower contamination risk
o No differences between batches
12. What is a diastema?
o Gap between the molars and incisors
13. How to prepare an operation (pre-operative prevention)?
o Inside out disinfect (in circle motion) the animal
o Outside in disinfect the animal
o With triangular shaving
o Drape must be used on the animal and the rest of your equipment
14. What is the optimal water temperature for zebrafish (danio rerio)?
o 27-29°C (28,5°C)
15. What is redirected behavior (conflict behavior)?
o Aggression shifts from the initial object toward other objects
o More playful behaviour
o Aggression to a new object
o Stereotypical behaviour
16. What are the steps of health screening next to pre-necropsy and necropsy?
o Bacteriology, serology and parasitology
o PCR and indirect methods
o PCR, serology and parasitology
17. Why do you perform pilot studies?
o To look at the feasibility and refine the experiment
o To decrease the number of animals used
18. Wat is de meest voorkomende bijwerking van NSAID’s gebruiken bij proefdieren?
o Gastric ulcers
19. Welke medicatie wordt als alfa-adrenerge agonist gebruikt?
o Metaraminol (ook: xylazine en medetomidine)
20. Welke medicatie wordt als lokaal anestheticum gebruikt bij muizen?
o Xylocaine (lidocaine)
21. Wat is de gestatie periode van een oryctolagus cuniculus (konijn)?
o 30-35 dagen (31 dagen)
22. In een experiment gaan zebravissen dood. Wat is de meest waarschijnlijke reden?
o Slechte water kwaliteit
2
, 23. Wat is een manier om allergieën te voorkomen?
o Investeer in ingenieurtechnieken zoals goede ventilatie en filtratie
24. Wat is het grote voordeel van F1-hybrids (inbred strains)?
o Bezitten meer ‘hybrid vigor’ dan hun ouders
o Makkelijker te fokken
25. What are gnotobionts?
o Condition of animals that are germ-free or have a known and controlled
set of microorganisms living in or on them (harbour a fully known
microflora)
26. Waarvoor gebruik je een non-technical summary (NTS)?
o An NTS is a brief summary of an animal research project written in
clear, accessible language for the general public
27. Wat doet een animal welfare body (AWB) niet?
o Conducting animal experiences
o Supervision
o Opvolgen van approval
o Make sure the 3 R's are known by everyone
28. Wat is de impact van chronisch stress op de hippocampus bij muizen?
o Daling neurogenese
o Stijging neurogenese
o Geen
29. Wat is het voordeel van inhalatie anesthesie(t.o.v. injectie anesthesie)?
o Precise control over the depth/duration of anesthesia an fast recovery
o Less equipment
o Cheaper
o All of the above
30. Wat is de reden dat dieren ziek worden?
o All of the above
o Slecht omgeving
o Pathogenen
31. Wat is de moeilijkheid om inbred muizen consistent te houden?
o Genetisch drift
32. Wat is het gevolg als je muizen sociaal onopgelost conflict hebben (environmental
factors that disturb the homeostasis when an animal cannot adapt)?
o Stress (and decreased well-being)
33. Casus: Je hebt varkens waarbij je ischemie gaat induceren, ze krijgen de correcte
anaesthesie. Na de operatie krijgen ze 1 week analgesie. Vervolgens wordt er een
product (medicatie) ingespoten om te kijken of dit zou helpen. Gedurende 3 maanden
worden ook ECG’s afgenomen. Wat is de severity van dit onderzoek?
o Moderate (surgery and post-operative pain)
o Mild
o Post-mortem
o Non-recovery
34. What hormone is released fast when the animal experiences acute stress?
o Cortisol (and ACTH)
o Estrogen
3
