AAB MT Chemistry Questions And Answers
With Complete Solution
Which of the four elements is proteins differentiates this class substances from
carbohydrates and lipids?
A. carbon
B. hydrogen
C. nitrogen
D. oxygen - CORRECT - ANSWERS C. Nitrogen
Which of the proteins is soluble in water?
A. albumin
B. alpha-globulins
C. beta-globulins
D. gamma-globulins - CORRECT - ANSWERS A. Albumin
How are amino acids joined together to form a chainlike structure?
A. disulfide bonding
B. hydrogen bonding
C. covalent bonding
D. peptide bonding - CORRECT - ANSWERS D. Peptide bonding
,Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
treatment. What does denaturation of proteins refer to?
A. amino acid substitution
B. addition of a prosthetic group
C. alteration in tertiary structure
D. increase in solubility - CORRECT - ANSWERS C. Alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency?
A. maple syrup substitution
B. phenylketonuria
C. alkaptonuria
D. cystinuria - CORRECT - ANSWERS D. Cystinuria
How many immunoglobulin classes are currently recognized?
A. two
B. three
C. four
D. five - CORRECT - ANSWERS D. Five
Which reagent is employed in the serum protein determination?
A. biuret
B. molybdenum blue
,C. ferricferrocyanide
D. diacetyl monoxime - CORRECT - ANSWERS A. Biuret
Why should hemolyzed serum not be used for total protein analysis?
A. hemoglobin is absorbed at the same wavelength as protein in the reagent
B. there are elevated protein amounts in red blood cells as compared to serum
C. hemoglobin reacts with biuret reagent
D. the value will be falsely lowered - CORRECT - ANSWERS A. Hemoglobin is absorbed at
the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction?
A. follows Beer's Law for a reasonable range of protein
B. depends on the presence of at least two peptide linkages
C. is relatively free from interference by lipids and hemoglobin
D. is insensitive to the low protein levels of urine - CORRECT - ANSWERS C. is relatively
free from interference by lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding techniques?
, A. it lends itself to manual and automated procedures
B. it never bind with alpha or beta globulins
C. it can be employed with both serum and plasma
D. there is less interference from pigment(s) - CORRECT - ANSWERS D. there is less
interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6?
A. albumin
B. alpha-globulins
C. beta-globulins
D. gamma-globulins - CORRECT - ANSWERS A. albumin
The total protein is 7.2. The serum protein results are as follows:
Albumin 53% 3.8 g/dL
Alpha-1 3.6% 0.3 g/dL
Alpha-2 11% 0.8 g/dL
Betta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio?
A. 3.4
With Complete Solution
Which of the four elements is proteins differentiates this class substances from
carbohydrates and lipids?
A. carbon
B. hydrogen
C. nitrogen
D. oxygen - CORRECT - ANSWERS C. Nitrogen
Which of the proteins is soluble in water?
A. albumin
B. alpha-globulins
C. beta-globulins
D. gamma-globulins - CORRECT - ANSWERS A. Albumin
How are amino acids joined together to form a chainlike structure?
A. disulfide bonding
B. hydrogen bonding
C. covalent bonding
D. peptide bonding - CORRECT - ANSWERS D. Peptide bonding
,Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
treatment. What does denaturation of proteins refer to?
A. amino acid substitution
B. addition of a prosthetic group
C. alteration in tertiary structure
D. increase in solubility - CORRECT - ANSWERS C. Alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency?
A. maple syrup substitution
B. phenylketonuria
C. alkaptonuria
D. cystinuria - CORRECT - ANSWERS D. Cystinuria
How many immunoglobulin classes are currently recognized?
A. two
B. three
C. four
D. five - CORRECT - ANSWERS D. Five
Which reagent is employed in the serum protein determination?
A. biuret
B. molybdenum blue
,C. ferricferrocyanide
D. diacetyl monoxime - CORRECT - ANSWERS A. Biuret
Why should hemolyzed serum not be used for total protein analysis?
A. hemoglobin is absorbed at the same wavelength as protein in the reagent
B. there are elevated protein amounts in red blood cells as compared to serum
C. hemoglobin reacts with biuret reagent
D. the value will be falsely lowered - CORRECT - ANSWERS A. Hemoglobin is absorbed at
the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction?
A. follows Beer's Law for a reasonable range of protein
B. depends on the presence of at least two peptide linkages
C. is relatively free from interference by lipids and hemoglobin
D. is insensitive to the low protein levels of urine - CORRECT - ANSWERS C. is relatively
free from interference by lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding techniques?
, A. it lends itself to manual and automated procedures
B. it never bind with alpha or beta globulins
C. it can be employed with both serum and plasma
D. there is less interference from pigment(s) - CORRECT - ANSWERS D. there is less
interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6?
A. albumin
B. alpha-globulins
C. beta-globulins
D. gamma-globulins - CORRECT - ANSWERS A. albumin
The total protein is 7.2. The serum protein results are as follows:
Albumin 53% 3.8 g/dL
Alpha-1 3.6% 0.3 g/dL
Alpha-2 11% 0.8 g/dL
Betta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio?
A. 3.4
