MCB 150 EXAM QUESTIONS WITH COMPLETE
SOLUTIONS
2.Explain the differences between cell specification, cell
determination and cell
differentiation Correct Answers the cell or tissue is capable of
differentiating autonomously (i.e. by itself) when placed in an
environment that is neutral with respect to the developmental
pathway irreversible, stage of cell or tissue
commitment in which the cell or tissue is capable of
differentiation autonomously even when placed into a non-
neutral environment.
The process by which an unspecialized cell becomes
specialized into one of many cell types that make up the body
2)Describe the species-specific basis of sperm-oocyte attraction,
the acrosome reaction and binding of the sperm acrosome to the
egg surface Correct Answers Chemoattraction B. Induction of
acrosome reaction C. Sperm binding to egg envelop D. Passage
of sperm through egg envelope E. Gamete fusionfusion of the
outer acrosomal membrane with the sperm plasma membrane
releases proteases that digest the egg coverings and exposes
proteins on the inner acrosomal membrane that bind to the egg
surface.
2a)Identify a testable hypothesis, apply rules of evidence to
design experimental tests, Correct Answers neccesitity for lose
it evidence was taken as we knocked out the gene and it
confirmed the progeny were eyeless.
, plausibility( this is a technique to look at from mrna
expression)We make rna probe complimentary to the rna we
want to detect using waxcrek base pair
We do it in a place where the antibody recognizes
Then hybridize to the tissue then if it find the mrna and forms
the Watson crek base pair
Now you can detect where they hybrids are Using the antibody
that detect that antigene
Add soluble substrate and spits out the insoluble colored mrn
you know those cells contain that particular rna. and since the
gene is expressed specifically in the eye that produce eye, thus it
is plausible.
suffieciency, in this case we want to move it in some other
tissue normally does not make eye and see it makes eye. in
doing so the promotoer was identified and expressed in all
imaginary discs including legs, antena. and using the promotor
the eyeless gene is hooked and all the disc turned the gene on
and expressed in many locations,such as legs ,antena and thus it
is sufficient.
3,Explain how Gurdon's experimental results suported the
"genomic equivalence"
hypothesis Correct Answers The theory that all cells of an
organism contain an equivalent complement of genetic
information. Genomic equivalence has been confirmed for most
cells,John Gurdon's nuclear transplantation experiments in frogs
- differentiated intestinal epithelial cells can be reprogrammed in
an enucleated egg to produce new individuals
3.Define what is meant by the "maternal-to-zygotic transition"
and describe the evidence that early
SOLUTIONS
2.Explain the differences between cell specification, cell
determination and cell
differentiation Correct Answers the cell or tissue is capable of
differentiating autonomously (i.e. by itself) when placed in an
environment that is neutral with respect to the developmental
pathway irreversible, stage of cell or tissue
commitment in which the cell or tissue is capable of
differentiation autonomously even when placed into a non-
neutral environment.
The process by which an unspecialized cell becomes
specialized into one of many cell types that make up the body
2)Describe the species-specific basis of sperm-oocyte attraction,
the acrosome reaction and binding of the sperm acrosome to the
egg surface Correct Answers Chemoattraction B. Induction of
acrosome reaction C. Sperm binding to egg envelop D. Passage
of sperm through egg envelope E. Gamete fusionfusion of the
outer acrosomal membrane with the sperm plasma membrane
releases proteases that digest the egg coverings and exposes
proteins on the inner acrosomal membrane that bind to the egg
surface.
2a)Identify a testable hypothesis, apply rules of evidence to
design experimental tests, Correct Answers neccesitity for lose
it evidence was taken as we knocked out the gene and it
confirmed the progeny were eyeless.
, plausibility( this is a technique to look at from mrna
expression)We make rna probe complimentary to the rna we
want to detect using waxcrek base pair
We do it in a place where the antibody recognizes
Then hybridize to the tissue then if it find the mrna and forms
the Watson crek base pair
Now you can detect where they hybrids are Using the antibody
that detect that antigene
Add soluble substrate and spits out the insoluble colored mrn
you know those cells contain that particular rna. and since the
gene is expressed specifically in the eye that produce eye, thus it
is plausible.
suffieciency, in this case we want to move it in some other
tissue normally does not make eye and see it makes eye. in
doing so the promotoer was identified and expressed in all
imaginary discs including legs, antena. and using the promotor
the eyeless gene is hooked and all the disc turned the gene on
and expressed in many locations,such as legs ,antena and thus it
is sufficient.
3,Explain how Gurdon's experimental results suported the
"genomic equivalence"
hypothesis Correct Answers The theory that all cells of an
organism contain an equivalent complement of genetic
information. Genomic equivalence has been confirmed for most
cells,John Gurdon's nuclear transplantation experiments in frogs
- differentiated intestinal epithelial cells can be reprogrammed in
an enucleated egg to produce new individuals
3.Define what is meant by the "maternal-to-zygotic transition"
and describe the evidence that early
