MCB 2050: REGULATION OF GENE EXPRESSION IN
EUKARYOTES QUESTIONS WITH COMPLETE
SOLUTIONS
_______ __________ is the mechanism of equalizing the
activity of X-linked genes in organisms with XX/XY or XX/XO
sex-determination. Dosage compensation is an example of
chromosomewide regulation that occurs by different
mechanisms in different organisms. Correct Answers Dosage
compensation
_________ Acetylate histone lysine residues resulting in
loosening of DNA - nucleosome interaction - more open
chromatin facilitates TF binding and transcriptional activation.
Correct Answers HATs
___________ allow different types of cells, in multicellular
eukaryotes, to signal each other. Correct Answers hormones
___________ are proteins that bind to a sequence within the
promoter to facilitate proper alignment of RNA polymerase II
on the template strand of DNA. Correct Answers basal
transcription factors
___________ demonstrate binding of TF (or other DNA binding
protein) to specific chromatin location in vivo. Correct Answers
ChIP
_________________ is an Abnormal gene expression caused by
moving a gene from an euchromatic to a heterochromatic region
by a translocation or change in orientation by inversion. Can
,create a mixture of normal and mutant characteristics in the
same individual. Correct Answers Position-effect variegation
_________________ motif is a 2 alpha helices separated by a
short loop that form dimers through interaction of the helical
regions on each polypeptide. Correct Answers helix-loop-helix
_________________ removes the positive charge of the lysine
residues and thereby decreases the interaction between the
histone tails and the negatively charged DNA. Correct Answers
Acetylation
A ________________ is a motif that forms a short peptide loop
when two cysteines in one part of the polypeptide and two
histidines in another part nearby jointly bind a zinc ion. Correct
Answers zinc finger
A ________________ motif is a stretch of amino acids with a
leucine in
every 7th position, dimerization by interactions between
leucine residues. Correct Answers leucine-zipper
A pattern in a protein structure associated with its secondary
structure is called a: Correct Answers Motif
A signaling molecule produced and secreted into the blood
stream where it travels to and affects target cells some distance
from site of synthesis is called: Correct Answers Endocrine
signaling
, A small, noncoding RNA molecule, typically about 21 bases
long, that binds to mRNA to inhibit its translation. Correct
Answers microRNA
An autonomously folding functional module of a protein usually
with several motifs is called a: Correct Answers Domain
Basal TFs and general TFs: bind to ____ ______ sequences.
- __________: TATA binding protein (TBP) and several TFIIs
- __________: control constitutive gene expression: NF1, SP1
_________ : bind to enhancer sequences and regulate
transcription
- ___________: HSF1, steroid hormone receptors etc. Correct
Answers basal promoter; General TFs; Basal TFs; Activators;
inducible expression
Both embryonic cells and cancer cells divide quickly. How can
these two types of cells be distinguished from each other?
Correct Answers Cancer cells do not display contact inhibition
—they pile up on top of each other—whereas embryonic cells
spread out in flat sheets. Cancer cells are frequently aneuploid;
embryonic cells are euploid.
Chromatin Immunoprecipitation (ChIP) demonstrate binding of
____ (or other DNA-binding protein) to specific chromatin
location in vivo.
1. Cells treated with ______ to cross-link proteins and DNA.
2. DNA _____ and mixed with antibody (Ab) to specific TF.
3. DNA/TF/Ab complexes ________,
EUKARYOTES QUESTIONS WITH COMPLETE
SOLUTIONS
_______ __________ is the mechanism of equalizing the
activity of X-linked genes in organisms with XX/XY or XX/XO
sex-determination. Dosage compensation is an example of
chromosomewide regulation that occurs by different
mechanisms in different organisms. Correct Answers Dosage
compensation
_________ Acetylate histone lysine residues resulting in
loosening of DNA - nucleosome interaction - more open
chromatin facilitates TF binding and transcriptional activation.
Correct Answers HATs
___________ allow different types of cells, in multicellular
eukaryotes, to signal each other. Correct Answers hormones
___________ are proteins that bind to a sequence within the
promoter to facilitate proper alignment of RNA polymerase II
on the template strand of DNA. Correct Answers basal
transcription factors
___________ demonstrate binding of TF (or other DNA binding
protein) to specific chromatin location in vivo. Correct Answers
ChIP
_________________ is an Abnormal gene expression caused by
moving a gene from an euchromatic to a heterochromatic region
by a translocation or change in orientation by inversion. Can
,create a mixture of normal and mutant characteristics in the
same individual. Correct Answers Position-effect variegation
_________________ motif is a 2 alpha helices separated by a
short loop that form dimers through interaction of the helical
regions on each polypeptide. Correct Answers helix-loop-helix
_________________ removes the positive charge of the lysine
residues and thereby decreases the interaction between the
histone tails and the negatively charged DNA. Correct Answers
Acetylation
A ________________ is a motif that forms a short peptide loop
when two cysteines in one part of the polypeptide and two
histidines in another part nearby jointly bind a zinc ion. Correct
Answers zinc finger
A ________________ motif is a stretch of amino acids with a
leucine in
every 7th position, dimerization by interactions between
leucine residues. Correct Answers leucine-zipper
A pattern in a protein structure associated with its secondary
structure is called a: Correct Answers Motif
A signaling molecule produced and secreted into the blood
stream where it travels to and affects target cells some distance
from site of synthesis is called: Correct Answers Endocrine
signaling
, A small, noncoding RNA molecule, typically about 21 bases
long, that binds to mRNA to inhibit its translation. Correct
Answers microRNA
An autonomously folding functional module of a protein usually
with several motifs is called a: Correct Answers Domain
Basal TFs and general TFs: bind to ____ ______ sequences.
- __________: TATA binding protein (TBP) and several TFIIs
- __________: control constitutive gene expression: NF1, SP1
_________ : bind to enhancer sequences and regulate
transcription
- ___________: HSF1, steroid hormone receptors etc. Correct
Answers basal promoter; General TFs; Basal TFs; Activators;
inducible expression
Both embryonic cells and cancer cells divide quickly. How can
these two types of cells be distinguished from each other?
Correct Answers Cancer cells do not display contact inhibition
—they pile up on top of each other—whereas embryonic cells
spread out in flat sheets. Cancer cells are frequently aneuploid;
embryonic cells are euploid.
Chromatin Immunoprecipitation (ChIP) demonstrate binding of
____ (or other DNA-binding protein) to specific chromatin
location in vivo.
1. Cells treated with ______ to cross-link proteins and DNA.
2. DNA _____ and mixed with antibody (Ab) to specific TF.
3. DNA/TF/Ab complexes ________,
