MCB 2050 Midterm Questions With Complete Solutions
After two rounds of PCR how many target copies of dsDNA are
produced? Correct Answers ? --> 2^n
cDNA has the same sequence as its template i) ________ but
consists of ii) ________. Correct Answers i) mRNA; ii) dNTPs
G- C base pairs contain i) _______ hydrogen bonds, while A-T
pairs contain ii)_______. Correct Answers i) 3; ii) 2
How do ddNTPs terminate chain synthesis in during Sanger
Sequencing? Correct Answers Once a ddNTP is incorporated
no additional dNTPs can be added because the 3'-OH group
required for DNA polymerase function is missing
How many hydrogen bonds occur in the following double
stranded DNA fragment?
5'ATGGTT 3'
3'TACCAA 5' Correct Answers 14
How many polypeptides combine to create the complete
antibody molecule? Correct Answers
If all of our cells contain the same DNA how is the variability
between cell types, neurons vs. skins cells for example,
produced? Correct Answers Altered gene expression
In a quantitative PCR (qPCR) reaction a high CT value indicates
i) ______ levels of target ii) _______ in the original sample.
Correct Answers
, In a quantitative PCR (qPCR) reaction a low CT value indicates
i) ______ levels of target ii) _______ in the original sample.
Correct Answers
In a quantitative PCR (qPCR) reaction the cycle at which the
fluorescent signal is detectable above background levels is
called: Correct Answers Threshold Cycle (CT)
In a quantitative reverse transcription PCR (qRT-PCR) reaction
a high CT value indicates i) ______ levels of target ii) _______
in the original sample. Correct Answers
In an RT-PCR reaction what type of molecule would be targeted
if non-specific primers are used? Correct Answers
In an RT-PCR reaction what type of molecule would be targeted
if polyT primers are used? Correct Answers Total mRNA
In contrast to traditional PCR, Quantitative PCR (qPCR):
Correct Answers monitors the PCR product throughout the
reaction.
In order for two DNA/RNA strands to hybridization they must:
Correct Answers have complimentary sequences
In PCR the forward primer anneals to the i) ________ strand
and has the same sequence as the ii) ________ strand. Correct
Answers bottom, top
After two rounds of PCR how many target copies of dsDNA are
produced? Correct Answers ? --> 2^n
cDNA has the same sequence as its template i) ________ but
consists of ii) ________. Correct Answers i) mRNA; ii) dNTPs
G- C base pairs contain i) _______ hydrogen bonds, while A-T
pairs contain ii)_______. Correct Answers i) 3; ii) 2
How do ddNTPs terminate chain synthesis in during Sanger
Sequencing? Correct Answers Once a ddNTP is incorporated
no additional dNTPs can be added because the 3'-OH group
required for DNA polymerase function is missing
How many hydrogen bonds occur in the following double
stranded DNA fragment?
5'ATGGTT 3'
3'TACCAA 5' Correct Answers 14
How many polypeptides combine to create the complete
antibody molecule? Correct Answers
If all of our cells contain the same DNA how is the variability
between cell types, neurons vs. skins cells for example,
produced? Correct Answers Altered gene expression
In a quantitative PCR (qPCR) reaction a high CT value indicates
i) ______ levels of target ii) _______ in the original sample.
Correct Answers
, In a quantitative PCR (qPCR) reaction a low CT value indicates
i) ______ levels of target ii) _______ in the original sample.
Correct Answers
In a quantitative PCR (qPCR) reaction the cycle at which the
fluorescent signal is detectable above background levels is
called: Correct Answers Threshold Cycle (CT)
In a quantitative reverse transcription PCR (qRT-PCR) reaction
a high CT value indicates i) ______ levels of target ii) _______
in the original sample. Correct Answers
In an RT-PCR reaction what type of molecule would be targeted
if non-specific primers are used? Correct Answers
In an RT-PCR reaction what type of molecule would be targeted
if polyT primers are used? Correct Answers Total mRNA
In contrast to traditional PCR, Quantitative PCR (qPCR):
Correct Answers monitors the PCR product throughout the
reaction.
In order for two DNA/RNA strands to hybridization they must:
Correct Answers have complimentary sequences
In PCR the forward primer anneals to the i) ________ strand
and has the same sequence as the ii) ________ strand. Correct
Answers bottom, top
