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MICROBIOLOGY MODULE 5 PORTAGE LEARNING COURSE // A+ GRADED PRESENTATION // ADVANCED MICROBIAL PHYSIOLOGY PATHOGENESIS IMMUNOLOGY AND LAB APPLICATIONS FOR STUDENT EXCELLENCE // ACADEMIC YEAR 2025/2026

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MICROBIOLOGY MODULE 5 PORTAGE LEARNING COURSE // A+ GRADED PRESENTATION // ADVANCED MICROBIAL PHYSIOLOGY PATHOGENESIS IMMUNOLOGY AND LAB APPLICATIONS FOR STUDENT EXCELLENCE // ACADEMIC YEAR 2025/2026

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MICROBIOLOGY MODULE 5 PORTAGE LEARNING COURSE
// A+ GRADED PRESENTATION // ADVANCED MICROBIAL
PHYSIOLOGY PATHOGENESIS IMMUNOLOGY AND LAB
APPLICATIONS FOR STUDENT EXCELLENCE // ACADEMIC
YEAR 2025/2026


CLSI (Clinical and Laboratory Standards Institute) - THE CORRECT
ANSWER -Up-to-date tables that list potential antimicrobial agents to
include in batteries for testing against particular organisms or organism
groups is published by:
-CDC (Centers for Disease Control and Prevention)
-NCCLS (National Committee for Clinical Laboratory Standards)
-MSOP (Manual of Standard Operating Procedures)
-CLSI (Clinical and Laboratory Standards Institute)


the lowest antimicrobial agent concentration that completely inhibits
visible bacterial growth - THE CORRECT ANSWER -The minimum
inhibitory concentration (MIC) result recorded in antimicrobial testing can
be defined as:
-the maximum concentration of antimicrobial agent that should be used
for optimal therapy
-the minimum concentration of antimicrobial agent that should be used
for optimal therapy
-the lowest antimicrobial agent concentration that completely inhibits
visible bacterial growth
-the highest antimicrobial agent concentration that completely inhibits
visible bacterial growth


Mueller-Hinton agar - THE CORRECT ANSWER -In the disk diffusion
method of antimicrobial susceptibility testing, what is the standard agar
base medium for testing most bacterial organisms?
-5% blood agar

, -Mueller-Hinton agar
-Mueller-Hinton broth
-Brain heart infusion broth
-any of the above can be used


the clinical significance of a bacterial isolate, the predictability of a
bacterial isolate's susceptibility to the antimicrobial agents most
commonly used against them, availability of reliable standardized
methods for testing the isolate - THE CORRECT ANSWER -The
decision to perform susceptibility testing depends on:
-the clinical significance of a bacterial isolate
-the predictability of a bacterial isolate's susceptibility to the antimicrobial
agents most commonly used against them
-availability of reliable standardized methods for testing the isolate
-the clinical significance of a bacterial isolate, the predictability of a
bacterial isolate's susceptibility to the antimicrobial agents most
commonly used against them, availability of reliable standardized
methods for testing the isolate


detection and/or the abundance of the organism on direct specimen
Gram stain in the presence of white blood cells - THE CORRECT
ANSWER -Criteria for establishing a bacterial isolate as clinically
significant include:
-cultivation of the organism from a clinical specimen
-detection and/or the abundance of the organism on direct specimen
Gram stain in the presence of white blood cells
-predominance of the organism in specimen cultures
-its antimicrobial susceptibility results


use of a pure culture - THE CORRECT ANSWER -An important
requirement for appropriate inoculum preparation in susceptibility testing
includes:
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