Chapter 8 Test Bank Questions with
Guaranteed Pass Solutions 2025-2026.
Sterilization - Answer removal or destruction of all forms of microbial life
Disinfection - Answer removing pathogens from nonliving material
Antisepsis - Answer removing pathogens from living tissue
Degerming - Answer antisepsis of a limited area; • ex. alcohol swab of skin b/f an injection
Sanitation - Answer disinfection of eating & drinking utensils; reduces the # of microbes
Germicide - Answer a method or agent which kills microbes
Bacteriostasis - Answer a method or agent which inhibits microbial growth
Sepsis - Answer microbial contamination
-cide - Answer An agent that kills microbes (e.g., a fungicide, a bacteriocide)
Heating Methods - Answer Moist Heat & Dry Heat
actions of microbial agents - Answer alters membrane permeability, damages proteins,
damages nucleic acids
Moist Heat - Answer Boling, Autoclaving
Autoclaving - Answer steam under pressure, denatures proteins
, HTST (high temperature short time) - Answer 72o C 15 sec.; widely used for Milk
UHT (ultra high temp.) - Answer 140o C < 1 sec. followed by rapid cooling; can sterilize; often
used for half & half
dry heat - Answer kills by oxidation. Flaming, incineration
Hot air sterilization - Answer 170o C for 2 hours can sterilize
Dessication - Answer Drying , prevents meatbolism
Osmotic Pressure - Answer High concentrations of sugars or salts (plasmolysis)
High Pressure - Answer Applied to liquids, denatures proteins and carbohydrates
commercial sterilization - Answer Killing C. botulinum endospores, with heat
asepsis - Answer absence of significant contamination
factors that effect microbial treatment - Answer Number of microbes, Environment (organic
matter, temperature, biofilms),Time of exposure, Microbial characteristics
Thermal Death Point - Answer Lowest temperature at which all cells in a culture are killed in
10 min
Thermal Death Time - Answer Time during which all cells in a culture are killed
steam sterilization - Answer steam must contact item's surface
filtration - Answer HEPA and membrane