PHLEBOTOMY NHA EXAM 2025 UPDATED ACTUAL EXAM WITH CORRECT SOLUTIONS.
First in the order of draw. Used to check for infection in the bloodstream. Do not invert or spin. -
(ANSWER)First in the order of draw. Used to check for infection in the bloodstream. Do not invert or
spin.
Light blue top tube - (ANSWER)Second in the order of draw. Sodium Citrate tube, invert 3-4 times. The
tube must be filled fully. When using a butterfly needle, a discard tube must be used before fully filling a
light blue-topped tube. (used for coagulation tests.
Gold topped tube - (ANSWER)Third in the order of draw. Is a Gel, or a Serum tube. Do not invert, or spin.
Do not use gel tubes for toxicology or drug testing.
Red or Black topped tube - (ANSWER)Fourth in the order of draw. Serum tube with no Gel, or SST with
gel. Invert 5 times, allow to clot for 30 minutes, then spin for 10-15 minutes. Then pour into a transport
vial labeled with specimen type and patient ID.
Green or Tan topped tube - (ANSWER)Fifth in the order of draw. Heparin tube. Needs to be inverted 8-
10 times. Do not spin.
Lavender or Tan topped tube - (ANSWER)Sixth in the order of draw. EDTA tube. Invert 8-10 times. Do
not Spin.
Royal Blue tube - (ANSWER)Seventh in the order of draw. EDTA tube. Do not invert, do not spin. It can
occasionally be used the same as a red-topped tube.
Grey tube - (ANSWER)Eighth in the order of draw. Sodium Fluoride (Glucose) or Glycolic inhibitor. Invert
8-10 times. Do not spin.
Yellow topped tubes - (ANSWER)Ninth in the order of draw, this tube is to be drawn last. Citrate ACD. Do
not invert.
Serum Iron test - (ANSWER)Used to measure iron in serum to check iron levels. Red topped or tiger
topped SST tubes
, PHLEBOTOMY NHA EXAM 2025 UPDATED ACTUAL EXAM WITH CORRECT SOLUTIONS.
TBIC (total iron binding capacity) test - (ANSWER)Measures bloods ability to bind iron with transferrin.
Tested in red top or tiger topped SST tubes
Ferritin test - (ANSWER)Measures iron storage in the body. Red topped or tiger topped SST tubed
Reticulocyte count - (ANSWER)Evaluates red blood cell production. Tested in a lavender top EDTA tube.
CBC test - (ANSWER)Complete Blood Count- assesses overall blood health. Lavender top EDTA.
Hemoglobin A1C - (ANSWER)Monitors long-term glucose control. Lavender top EDTA
Fasting Glucose - (ANSWER)Measures Blood Sugar Levels. Grey top Sodium Fluoride tube.
PT/INR - (ANSWER)Evaluates blood clotting time. Light blue top Sodium Citrate
Lipid panel - (ANSWER)Assesses cholesterol and triglyceride levels. Tiger top SST.
Thyroid panel - (ANSWER)Measures thyroid function (TSH, T3, T4) Tiger top or red topped tubes.
Vitamin D - (ANSWER)Measures Vitamin D levels in blood. Tiger top or red topped tubes.
Hepatitis panel - (ANSWER)Detects hepatitis A, B , and/or C antibodies. Red top or tiger topped tubes.
HIV screen - (ANSWER)Screens for HIV infection. Red topped or tiger topped tubes
Blood type & RH - (ANSWER)Determines for blood group and Rh factor. Pink or lavender topped tubes.
First in the order of draw. Used to check for infection in the bloodstream. Do not invert or spin. -
(ANSWER)First in the order of draw. Used to check for infection in the bloodstream. Do not invert or
spin.
Light blue top tube - (ANSWER)Second in the order of draw. Sodium Citrate tube, invert 3-4 times. The
tube must be filled fully. When using a butterfly needle, a discard tube must be used before fully filling a
light blue-topped tube. (used for coagulation tests.
Gold topped tube - (ANSWER)Third in the order of draw. Is a Gel, or a Serum tube. Do not invert, or spin.
Do not use gel tubes for toxicology or drug testing.
Red or Black topped tube - (ANSWER)Fourth in the order of draw. Serum tube with no Gel, or SST with
gel. Invert 5 times, allow to clot for 30 minutes, then spin for 10-15 minutes. Then pour into a transport
vial labeled with specimen type and patient ID.
Green or Tan topped tube - (ANSWER)Fifth in the order of draw. Heparin tube. Needs to be inverted 8-
10 times. Do not spin.
Lavender or Tan topped tube - (ANSWER)Sixth in the order of draw. EDTA tube. Invert 8-10 times. Do
not Spin.
Royal Blue tube - (ANSWER)Seventh in the order of draw. EDTA tube. Do not invert, do not spin. It can
occasionally be used the same as a red-topped tube.
Grey tube - (ANSWER)Eighth in the order of draw. Sodium Fluoride (Glucose) or Glycolic inhibitor. Invert
8-10 times. Do not spin.
Yellow topped tubes - (ANSWER)Ninth in the order of draw, this tube is to be drawn last. Citrate ACD. Do
not invert.
Serum Iron test - (ANSWER)Used to measure iron in serum to check iron levels. Red topped or tiger
topped SST tubes
, PHLEBOTOMY NHA EXAM 2025 UPDATED ACTUAL EXAM WITH CORRECT SOLUTIONS.
TBIC (total iron binding capacity) test - (ANSWER)Measures bloods ability to bind iron with transferrin.
Tested in red top or tiger topped SST tubes
Ferritin test - (ANSWER)Measures iron storage in the body. Red topped or tiger topped SST tubed
Reticulocyte count - (ANSWER)Evaluates red blood cell production. Tested in a lavender top EDTA tube.
CBC test - (ANSWER)Complete Blood Count- assesses overall blood health. Lavender top EDTA.
Hemoglobin A1C - (ANSWER)Monitors long-term glucose control. Lavender top EDTA
Fasting Glucose - (ANSWER)Measures Blood Sugar Levels. Grey top Sodium Fluoride tube.
PT/INR - (ANSWER)Evaluates blood clotting time. Light blue top Sodium Citrate
Lipid panel - (ANSWER)Assesses cholesterol and triglyceride levels. Tiger top SST.
Thyroid panel - (ANSWER)Measures thyroid function (TSH, T3, T4) Tiger top or red topped tubes.
Vitamin D - (ANSWER)Measures Vitamin D levels in blood. Tiger top or red topped tubes.
Hepatitis panel - (ANSWER)Detects hepatitis A, B , and/or C antibodies. Red top or tiger topped tubes.
HIV screen - (ANSWER)Screens for HIV infection. Red topped or tiger topped tubes
Blood type & RH - (ANSWER)Determines for blood group and Rh factor. Pink or lavender topped tubes.
