OPENSTAX MICROBIOLOGY CHAPTER 13 EXAM
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Formites - .....ANSWER ...✔✔ any non-living object
that can be contaminated by microbes.
The Centers for Disease Control and Prevention (CDC)
and the National Institutes of Health (NIH) have
established 4 classification levels called ________. -
.....ANSWER ...✔✔ Biological Safety Levels (BSL)
BSL-1 - .....ANSWER ...✔✔ Microorganisms not known
to cause disease in healthy adults.
BSL-2 organisms - .....ANSWER ...✔✔ present a
moderate environmental and or health hazard, can
cause a disease but is treatable
BSL-3 organisms - .....ANSWER ...✔✔ have potential
to cause lethal infections by inhalation. Divert from
foreign location. Ex. HIV, West Nile Virus
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BSL-4 organisms - .....ANSWER ...✔✔ Most dangerous
and often fatal. These microbes are typically exotic, are
easily transmitted by inhalation and cause infectious for
which there's no treatment or vaccinations.
Autoclave - .....ANSWER ...✔✔ use moist heat, high
pressure steam to serialize.
Sterilization - .....ANSWER ...✔✔ removal of ALL
microbes.
- lab uses autoclaves to sterilize tubes and plates.
Asepsis - .....ANSWER ...✔✔ treatments that decrease
microbe numbers on LIVING TISSUES.
Aseptic technique - .....ANSWER ...✔✔ any procedure
that minimizes contamination to a patient.
- example: using sterile equipment and sterilizing or
disposing of contaminated equipment properly.
Antiseptic(s) - .....ANSWER ...✔✔ chemicals that
decrease microbe numbers.
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Commercial sterilization - .....ANSWER ...✔✔ kills
disease causing microbes, but not microbes that grow at
high temperatures.
does commercial sterilization truly sterilize? -
.....ANSWER ...✔✔ no, does not kill microbes that
grow at high temperatures.
What is commercial sterilization trying to eliminate and
for what reason? - .....ANSWER ...✔✔ intended to kill
clostridium botulinum endospores to prevent botulism
poisoning.
Which requires longer exposure to formites, disinfection
or sanitization? - .....ANSWER ...✔✔ disinfection
does disinfecting something achieve sterilization? -
.....ANSWER ...✔✔ no, does not destroy endospores.
does sanitizing something achieve sterilization -
.....ANSWER ...✔✔ no, does not destroy endospores.