ESTUDYR
UK PIL A/B/C TRAINING UOB MOST TESTED QUESTIONS
AND ANSWERS GRADED A+ WITH RATIONALES
What does ASPA stand for?
A. Animal Science Practice Act
B. ✔ Animals (Scientific Procedures) Act
C. Animal Safety & Protection Association
D. Animal Science & Policy Act
Rationale: ASPA is the UK law regulating scientific procedures on animals.
What do the ARRIVE guidelines address?
A. Animal transport standards
B. Laboratory ventilation limits
C. ✔ Reporting of in vivo experiments to maximise usefulness of results
D. Veterinary clinical practice standards
Rationale: ARRIVE set standards for reporting animal experiments to improve reproducibility.
What is the purpose of the PREPARE guidelines?
A. Regulate animal housing size
B. Publish animal welfare laws
C. ✔ Planning research and experimental procedures on animals (recommendations for
excellence)
D. Licence veterinary drugs
Rationale: PREPARE guides planning to ensure experiments are ethical and well designed.
Which is NOT one of the Five Freedoms?
A. Freedom from hunger and thirst
B. Freedom from discomfort
C. Freedom from pain, injury and disease
D. ✔ Freedom of unrestricted movement outdoors
Rationale: The Five Freedoms include welfare basics and freedom from fear, not unrestricted
outdoor access.
A “regulated procedure” is any procedure that may cause which of the following?
A. Minor grooming only
B. ✔ Pain, suffering, distress or lasting harm (includes withholding)
C. Only death
D. Only behavioural observation
,ESTUDYR
Rationale: Regulated procedures are defined by their potential to negatively affect welfare
beyond trivial effects.
The threshold for a procedure to be “regulated” is roughly equivalent to:
A. A paper cut
B. ✔ Pain/suffering greater than insertion of a hypodermic needle performed under good
veterinary practice
C. Routine feeding
D. Observational behaviour studies only
Rationale: The legal threshold compares to the pain of a standard needle insertion.
Which is an example of a non-regulated procedure?
A. Deliberately inducing disease for research
B. ✔ Marking for identification as part of normal husbandry
C. Withholding treatments in experiments
D. Performing an experimental biopsy
Rationale: Routine husbandry actions (identification marking) are generally non-regulated.
Are killing methods not in Schedule 1 ever permitted?
A. Never permitted under any circumstances
B. ✔ Yes, but require Secretary of State approval and justification that Schedule 1 would be
unsuitable
C. Allowed if more convenient
D. Only if the vet requests it orally
Rationale: Non-Schedule 1 killing requires high-level permission and scientific justification.
What is Schedule 2 about?
A. Approved anaesthetics list
B. ✔ Species that must be obtained from an ASPA-registered source (licensed
breeders/suppliers)
C. List of named persons
D. Housing standards
Rationale: Schedule 2 lists species that must come from licensed suppliers.
Which species are typically on Schedule 2?
A. Only farm livestock
B. ✔ Mice, rats, guinea pigs, hamsters, rabbits, dogs, cats, primates, zebrafish, GM
pigs/sheep, quail, ferrets, gerbils, frogs
C. Only cephalopods
, ESTUDYR
D. All wild animals
Rationale: Common laboratory species and many GM animals are Schedule 2.
Which animals are “protected” as adults under ASPA?
A. Only mammals and birds
B. Only lab-bred animals
C. ✔ Any living vertebrate (except humans) and any living cephalopod
D. Only Schedule 2 species
Rationale: Protection covers all vertebrates (and cephalopods) from defined developmental
stages.
For ASPA, an animal is considered “living” until:
A. It stops moving for a minute
B. ✔ Permanent cessation of circulation or destruction of the brain
C. Temperature falls below 20°C
D. Owner declares it dead
Rationale: Legal definitions require irreversible circulatory or brain cessation.
At what stage are mammals, birds and reptiles considered protected in utero/egg?
A. Immediately after fertilisation
B. At birth/ hatching only
C. ✔ In the last third of gestation/incubation (e.g., day 14 for mice/rats)
D. Only after weaning
Rationale: Protection begins late in gestation/incubation when developmental capacity for pain
rises.
When are fish and amphibians considered protected?
A. From fertilisation
B. From metamorphosis only
C. ✔ Once capable of independent feeding
D. At sexual maturity
Rationale: Independent feeding marks a developmental threshold for protection.
When are cephalopods protected?
A. At adult stage only
B. ✔ From hatching (as soon as they hatch)
C. After one week post-hatch
D. Only when they can mate
Rationale: Cephalopods are protected from hatching due to neural complexity and sentience
concerns.
UK PIL A/B/C TRAINING UOB MOST TESTED QUESTIONS
AND ANSWERS GRADED A+ WITH RATIONALES
What does ASPA stand for?
A. Animal Science Practice Act
B. ✔ Animals (Scientific Procedures) Act
C. Animal Safety & Protection Association
D. Animal Science & Policy Act
Rationale: ASPA is the UK law regulating scientific procedures on animals.
What do the ARRIVE guidelines address?
A. Animal transport standards
B. Laboratory ventilation limits
C. ✔ Reporting of in vivo experiments to maximise usefulness of results
D. Veterinary clinical practice standards
Rationale: ARRIVE set standards for reporting animal experiments to improve reproducibility.
What is the purpose of the PREPARE guidelines?
A. Regulate animal housing size
B. Publish animal welfare laws
C. ✔ Planning research and experimental procedures on animals (recommendations for
excellence)
D. Licence veterinary drugs
Rationale: PREPARE guides planning to ensure experiments are ethical and well designed.
Which is NOT one of the Five Freedoms?
A. Freedom from hunger and thirst
B. Freedom from discomfort
C. Freedom from pain, injury and disease
D. ✔ Freedom of unrestricted movement outdoors
Rationale: The Five Freedoms include welfare basics and freedom from fear, not unrestricted
outdoor access.
A “regulated procedure” is any procedure that may cause which of the following?
A. Minor grooming only
B. ✔ Pain, suffering, distress or lasting harm (includes withholding)
C. Only death
D. Only behavioural observation
,ESTUDYR
Rationale: Regulated procedures are defined by their potential to negatively affect welfare
beyond trivial effects.
The threshold for a procedure to be “regulated” is roughly equivalent to:
A. A paper cut
B. ✔ Pain/suffering greater than insertion of a hypodermic needle performed under good
veterinary practice
C. Routine feeding
D. Observational behaviour studies only
Rationale: The legal threshold compares to the pain of a standard needle insertion.
Which is an example of a non-regulated procedure?
A. Deliberately inducing disease for research
B. ✔ Marking for identification as part of normal husbandry
C. Withholding treatments in experiments
D. Performing an experimental biopsy
Rationale: Routine husbandry actions (identification marking) are generally non-regulated.
Are killing methods not in Schedule 1 ever permitted?
A. Never permitted under any circumstances
B. ✔ Yes, but require Secretary of State approval and justification that Schedule 1 would be
unsuitable
C. Allowed if more convenient
D. Only if the vet requests it orally
Rationale: Non-Schedule 1 killing requires high-level permission and scientific justification.
What is Schedule 2 about?
A. Approved anaesthetics list
B. ✔ Species that must be obtained from an ASPA-registered source (licensed
breeders/suppliers)
C. List of named persons
D. Housing standards
Rationale: Schedule 2 lists species that must come from licensed suppliers.
Which species are typically on Schedule 2?
A. Only farm livestock
B. ✔ Mice, rats, guinea pigs, hamsters, rabbits, dogs, cats, primates, zebrafish, GM
pigs/sheep, quail, ferrets, gerbils, frogs
C. Only cephalopods
, ESTUDYR
D. All wild animals
Rationale: Common laboratory species and many GM animals are Schedule 2.
Which animals are “protected” as adults under ASPA?
A. Only mammals and birds
B. Only lab-bred animals
C. ✔ Any living vertebrate (except humans) and any living cephalopod
D. Only Schedule 2 species
Rationale: Protection covers all vertebrates (and cephalopods) from defined developmental
stages.
For ASPA, an animal is considered “living” until:
A. It stops moving for a minute
B. ✔ Permanent cessation of circulation or destruction of the brain
C. Temperature falls below 20°C
D. Owner declares it dead
Rationale: Legal definitions require irreversible circulatory or brain cessation.
At what stage are mammals, birds and reptiles considered protected in utero/egg?
A. Immediately after fertilisation
B. At birth/ hatching only
C. ✔ In the last third of gestation/incubation (e.g., day 14 for mice/rats)
D. Only after weaning
Rationale: Protection begins late in gestation/incubation when developmental capacity for pain
rises.
When are fish and amphibians considered protected?
A. From fertilisation
B. From metamorphosis only
C. ✔ Once capable of independent feeding
D. At sexual maturity
Rationale: Independent feeding marks a developmental threshold for protection.
When are cephalopods protected?
A. At adult stage only
B. ✔ From hatching (as soon as they hatch)
C. After one week post-hatch
D. Only when they can mate
Rationale: Cephalopods are protected from hatching due to neural complexity and sentience
concerns.
